prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

background: endometriosis is a disease that is hard to diagnose without the gold standard method, laparoscopy. an easier diagnostic method is needed. objective: the aim of the study is to determine whether the number of macrophage cells in the endometrium and/or the detection of nerve fibers can be used in the diagnosis of endometriosis. materials and methods: endometrial sampling was done to 3...

background: recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. integrin signaling may be regulated by a number of mechanisms including accessory proteins such as cd98 (4f2 antigen). objectives: to determine cd98 expression by human articular chondrocytes and its involvement in human articular mechano...

background although infection with helicobacter pylori is a global health problem, its prevalence is different among countries. serologic tests for the diagnosis of h. pylori infection are limited by low accuracy rates and lack of validation. recent studies indicate that the stool antigen test has an acceptable level of accuracy. objectives our objective was to investigate the prevalence of h. ...

background: the normal prostate-specific antigen (psa) level in patients with urethral catheterization is a controversy. we designed this study to investigate the effect of nontraumatic urethral catheterization without urinary retention on serum psa. materials and methods: seventy patients scheduled for urethral catheterization before elective surgeries were randomly selected and included in th...

the aim of this sutdy is to prepare histocompatability y(hy) antibody in female mouse at the first phase (current study), and to separate mouse embryos with regard to their sex, to be able to obtain offsprings of disired sex, by means of hy antibody, at the second phase. totally 421 balb/c inbred mice were used. antibodies were produced in females by intraperitonial injections of spleen and tes...

background: rh (rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. isolation of rhd from cord blod, cloning and expression of recombinant rhd antigen in bacterial expression system was the aim of this study. methods: total rnas were extracted from cord blood (o + ).  the quality of rna was determined by electrophoresis. in or­der to obtain coding sequen...

early diagnosis of human hydatid disease by detecting the specific antibodies in patients’ sera is considered as an important step in treatment of infection. but the diagnostic efficiencies of assays greatly depend on the characteristics of antigen that is used and various conditions in performance. in present study, we tried to standardize an indirect haemagglutination test, using antigen b fo...

background: visceral leishmaniasis is systematic serous parasitic disease with pub­lic health importance. zoonotic form of visceral leishmaniasis is wide spread in mediterranean basin and south america regions. direct agglutination test (dat) is an accurate, reliable and non-expensive serological test for the diagnosis of visceral leishmaniasis in human and canines but the antigen preparation i...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...