BACKGROUND Most insulin autoantibody assays for both human and animal models are in a radioassay format utilizing (125)I-insulin, but despite the radioassay format international workshops have documented difficulty in standardization between laboratories. There is thus a need for simpler assay formats that do not utilize radioactivity, yet retain the high specificity and sensitivity of radioass...

BACKGROUND Nanoparticle-based detection technologies have the potential to improve detection sensitivity in miniature as well as in conventional biochemical assays. We introduce a detection technology that relies on the use of europium(III) nanoparticles and time-resolved fluorometry to improve the detection limit of biochemical assays and to visualize individual molecules in a microtiter plate...

Normal prostate tissue contains high levels of citrate. In the presence of prostate cancer, the citrate level is diminished. In this paper we show that it is possible to use europium-oxytetracycline complex as a citrate fluorescent probe and consequently as a prostate cancer probe. We analyzed normal nude male mice urine and urine from nude male mice in which prostate cancer was induced by intr...

Rapid, sensitive and specific diagnostic assays play an indispensable role in determination of HIV infection stages and evaluation of efficacy of antiretroviral therapy. Recently, our laboratory developed a sensitive Europium nanoparticle-based microtiter-plate immunoassay capable of detecting target analytes at subpicogram per milliliter levels without the use of catalytic enzymes and signal a...

A novel europium(III) membrane luminescence sensor based on a tridentate bis(phosphinic amide)-phosphine oxide, PhPO(C(6)H(4)POPhN(CH(CH(3))(2))(2))(2) (1), is described. The new luminescent complex, [Eu(1)(2)]Cl(3)2, which is formed between europium(III) and ligand 1 and has a 1 : 2 stoichiometry, has been evaluated in solution. It has the excellent spectroscopic and chemical characteristics t...

Five rare earth complexeslanthanum(III),praseodymium(III),neodymium(III), samarium(III) and europium(III) have been synthesized from Schiff base ligand (N,N-bis (2-hydroxy-1-naphthylidene) acetylhydrazone). The complexes were characterized based on elemental analysis, molar conductance, ultraviolet, infrared, mass, thermogravimetric and powder X-ray diffraction studies. Infrared spectra sug...

High-resolution x-ray powder-diffraction experiments were performed on europium metal at high pressure up to 50 GPa. At variance with previous reports, the hcp phase of Eu was observed to be stable not only to 18 GPa, but to 31.5 GPa. At 31.5(5) GPa, europium transforms to a phase (Eu-IV) with an incommensurately modulated monoclinic crystal structure with superspace group C2/c(q(1)0q(3))00. Th...

The photoluminescence (PL) spectrum of KBr0.95 I0.05:Eu 2+ (0.05mol %) mixed crystal gives emission of europium at 420nm. This 420nm emission is due to the transition from T 2g component of 4f 6 5d configuration to the ground state 8s of europium. When adding Tl + ion as co-activator in KBr0.95 I0.05:Eu 2+ (0.05mol %) mixed crystal the same PL emission observed and enhanced their PL intensity. ...

In this work, Fourier Transform Infrared spectroscopy (FT-IR) analysis were conducted on europium doped hydroxyapatite, Ca10-xEux(PO4)6(OH)2 nanocrystalline powders (Eu:HAp) with 0 ≤ xEu ≤ 0.2. Antimicrobial studies were also performed for the first time on Eu:Hap. The antimicrobial properties of Eu:HAp nanoparticles with 0 ≤ xEu ≤ 0.2 on Gram-negative (E coli ATCC 25922, Pseudomonas aeruginosa...