نتایج جستجو برای: eukaryotic expression

تعداد نتایج: 898404  

Journal: Vaccine Research 2015
A Bolhassani , N Davoudi , Sh Irani, SM Mirnurollahi ,

Introduction: Hepatitis C virus (HCV) infection is a major public health problem which influences about 170 million people worldwide. Different types of vaccines have been designed using HCV structural proteins to control the viral infection. The core nucleocapsid protein is one of the most conserved proteins and a desirable target for HCV vaccines, especially the protein-based vaccines. In cur...

ژورنال: دانشور پزشکی 2013
بنده‌پور, مژگان, خرمگاه, مریم سادات, ضرغامی, نصرت‌اله, عباس‌زاده, حجت‌اله, کاظمی, بهرام,

Background and Objective: The glycoprotein hormone secreted from anterior pituitary gland are heterodimeric, non- covalently consisting of a common α subunit and a hormone-specific β subunit. Due to the lack of adequate access to hormones and the possibility of contamination, this study attempted to clone two subunits of LH hormone by using IRES sequence in eukaryotic expression vector pEGFP-NI...

Behnaz Forouhar Kalkhoran, Farida Behzadian, Farzaneh Sabahi, Hesam Mirshahabi, Mohsen Karimi,

Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...

Journal: :avicenna journal of medical biotechnology 0

background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

ژورنال: یافته 2014
احمدی, الهام , سلیمان جاهی, حوریه , مشکات, زهرا , میرشهابی, حسام ,

Background : The aim of the present study was to identify a suitable cell line for studies of recombinant protein production in eukaryotic system. After transfection, altered expression levels of RNA and its target protein were analyzed. Materials and Methods: To investigate the in vitro expression of E6 protein of human papillomavirus type 16 in cell culture, the plasmid pcDNA3-E6, and two di...

1999

Existing conditional expression systems can be classified in two major categories that are based either on the induction or on the de-repression of transcription. The system described here combines both mechanisms, since a unique transcription factor can be shifted from a repression to a stimulation activity by simply changing its ligand. The resulting advantage of this system is the complete a...

Journal: :vaccine research 0
sm mirnurollahi department of biology, science and research branch, islamic azad university, tehran, iran. sh irani department of biology, science and research branch, islamic azad university, tehran, iran. n davoudi department of biotechnology, pasteur institute of iran, tehran, iran. a bolhassani department of hepatitis and aids, pasteur institute of iran, tehran, iran

introduction: hepatitis c virus (hcv) infection is a major public health problem which influences about 170 million people worldwide. different types of vaccines have been designed using hcv structural proteins to control the viral infection. the core nucleocapsid protein is one of the most conserved proteins and a desirable target for hcv vaccines, especially the protein-based vaccines. in cur...

Journal: :archives of razi institute 2016
f. fotouhi m.h. roostaee h. soleimanjahi g.r. haqshenash m. jamalidoust

to construct of an eukaryotic expression vector encoding herpes simplex virus type 2 (hsv-2) glycoprotein d (gd2), an iranian isolate of hsv-2 was propagated in hela cell line and its dna was extracted and used as template in polymerase chain reactions (pcr), to amplify gd2 gene. primers were designed and the restriction enzyme sites for ecori and xhoi were considered at their 5′ ends respectiv...

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