objective: breast cancer is the most common cancer in iranian women. breast tumors are classified based on the estrogen receptor alpha (erα) expression status into er negative and er positive tumors. er negative tumors tend to have worse prognosis and less likely to respond to endocrine therapy. aberrant methylation of gene promoter is one of the mechanisms for gene silencing in breast tumors. ...

Transcription of the proto-oncogene c-fos is stimulated by 17 beta-estradiol in estrogen responsive human and rat cells. To understand the molecular mechanisms of estrogen regulation of c-fos gene transcription, the human c-fos gene promoter, with 2.25 Kb of 5'-flanking DNA, was cloned upstream of the bacterial CAT gene and tested for estrogen regulation by transient transfection in HeLa cells....

The incidence of reproductive disorders is constantly increasing and affects 15% couples, with male's abnormalities diagnosed in almost half the cases. male gonads exert two major functions testis productions gametes (exocrine function) sexual hormones (endocrine function). In last decades, next to steroid receptors such as estrogen androgen receptors, involvement other members nuclear receptor...

Mice with targeted gene disruption of one of the estrogen receptor coactivators, p300/CBP-associated factor (PCAF), and its counterpart, PCAF-B, were used to investigate the possible involvement of PCAF and PCAF-B in estrogen receptor-mediated actions in vivo. Among ovariectomized mice that were treated with estrogen, PCAF and PCAF/PCAF-B knockouts showed abnormal growth of the uterus compared ...

Objective(s): Estrogen receptor-alpha (ERα) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. It has a key role in spermatogenesis. Estrogen receptor alpha knock-out (ER koα) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. Recently, it has been reported that microRNA (miRNA) mir-100 and ...

abstract background: the aims of this study were to assay the activity of adenosine deaminase (ada) in estrogen receptor positive (mcf-7) and negative (mda-mb468) breast cancer cell lines. methods: mda-mb468 and mcf-7 breast cancer cell lines were cultured in complete medium, striped serum with and without 0.0 1~-lm diethylstilbestrol (des), complete medium in the presence and absence of 111m t...

Estrogen has pleiotropic effects on the cardiovascular system. The mechanisms by which estrogen confers these pleiotropic effects are undergoing active investigation. Until a decade ago, all estrogen signaling was thought to occur by estrogen binding to nuclear estrogen receptors (estrogen receptor-α and estrogen receptor-β), which bind to DNA and function as ligand-activated transcription fact...

The effects of cadmium on estrogen receptor and other estrogen-regulated genes in the human breast cancer cell line MCF-7 were studied. Treatment of MCF-7 cells with 1 microM cadmium decreased the level of estrogen receptor 58%. Cadmium induced a parallel decrease in estrogen receptor mRNA (62%). Progesterone receptor levels increased 3.2-fold after cadmium treatment. This induction was blocked...

This study is an attempt to determine whether estrogen could directly regulate human gonadotropin-releasing hormone (GnRH) gene expression. Human GnRH expression vectors were constructed by fusing various 5' flanking regions of the human GnRH gene upstream of the luciferase reporter gene (LUC) or the thymidine kinase promoter linked to the chloramphenicol acetyltransferase reporter gene (CAT). ...