نتایج جستجو برای: estexpressed sequence tags

تعداد نتایج: 419314  

2007
Irina Fedulova Zheng Ouyang Charles Buck Xiang Zhang

In recent years a number of de novo sequencing software products became available providing possible partial or complete amino acid sequence tags for MS/MS spectra of peptides. However, for a variety of reasons including spectral chemical noise and imperfect fragmentation these sequence tags almost always contain errors. Additional difficulties arise from actual protein sequence variation and p...

2011
Yutao Liu Drew Munro David Layfield Andrew Dellinger Jeffrey Walter Katherine Peterson Catherine Bowes Rickman R. Rand Allingham Michael A. Hauser

PURPOSE To identify the genes expressed in normal human trabecular meshwork tissue, a tissue critical to the pathogenesis of glaucoma. METHODS Total RNA was extracted from human trabecular meshwork (HTM) harvested from 3 different donors. Extracted RNA was used to synthesize individual SAGE (serial analysis of gene expression) libraries using the I-SAGE Long kit from Invitrogen. Libraries wer...

Journal: :Bioinformatics 2000
James A. Cuff Ewan Birney Michele E. Clamp Geoffrey J. Barton

MOTIVATION An automatic sequence searching method (ProtEST) is described which constructs multiple protein sequence alignments from protein sequences and translated expressed sequence tags (ESTs). ProtEST is more effective than a simple TBLASTN search of the query against the EST database, as the sequences are automatically clustered, assembled, made non-redundant, checked for sequence errors, ...

Journal: :Methods in molecular biology 2004
John Parkinson Mark Blaxter

Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database submission. These tools also can be used to ...

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