Essential medicines are those that satisfy the priority healthcare needs of the population and are available at all times in adequate amounts, in the appropriate dosage forms with assured quality and at a price the individual and community can afford. Essential medicines are selected with due regard to disease prevalence, efficacy, safety and cost effectiveness. In most developing countries, th...

Genes critical for the survival or reproduction of an organism in certain circumstances are classified as essential genes. Essential genes play a significant role in deciphering the survival mechanism of life. They may be greatly applied to pharmaceutics and synthetic biology. The continuous progress of experimental method for essential gene identification has accelerated the accumulation of ge...

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related bacteria. This extended the methodology commonly used to locate insertional mutations in individual mutants to the analysis of mutations in populatio...

let $varphi(z)=z^m, z in mathbb{u}$, for some positive integer $m$, and $c_varphi$ be the composition operator on the bergman space $mathcal{a}^2$ induced by $varphi$. in this article, we completely determine the point spectrum, spectrum, essential spectrum, and essential norm of the operators $c^*_varphi c_varphi, c_varphi c^*_varphi$ as well as self-commutator and anti-self-commutators of $c_...

On Oct 21, WHO published the full report of the 20th Expert Committee on the Selection and Use of Essential Medicines, with its new WHO Model List of Essential Medicines (EML). The new list includes recently developed medicines for drug-resistant tuberculosis (bedaquiline and delamanid), a number of new cancer treatments (such as imatinib, rituximab, and trastuzumab), and, perhaps most controve...

A protein depletion by promoter shutoff or protein destabilization is an important tool in investigation of functions of essential genes. Various approaches using different repressible promoters, inducible degrons, or their combinations were developed. While successful, the current techniques have a drawback in that they require fusion of a large degradation tag to the target protein and/or a c...

Variable screen quality, off-target effects, and unclear false discovery rates often hamper large-scale functional genomic screens in mammalian cells. Hart et al (2014) introduce gold standard reference sets of essential and non-essential genes, aiming at standardizing the analysis of genome-wide screens. This work provides a framework to compare both the quality and analysis methods of functio...