نتایج جستجو برای: esat6

تعداد نتایج: 122  

Journal: :Journal of immunology 2013
Susanna Commandeur Krista E van Meijgaarden Corine Prins Alexander V Pichugin Karin Dijkman Susan J F van den Eeden Annemieke H Friggen Kees L M C Franken Gregory Dolganov Igor Kramnik Gary K Schoolnik Fredrik Oftung Gro Ellen Korsvold Annemieke Geluk Tom H M Ottenhoff

Mycobacterium tuberculosis is responsible for almost 2 million deaths annually. Mycobacterium bovis bacillus Calmette-Guérin, the only vaccine available against tuberculosis (TB), induces highly variable protection against TB, and better TB vaccines are urgently needed. A prerequisite for candidate vaccine Ags is that they are immunogenic and expressed by M. tuberculosis during infection of the...

2015
Wandee Yindeeyoungyeon Kamolchanok Rukseree Siriporn Tungsudjai Tasanee Panichakul

The tuberculin Purified Protein Derivative (PPD) skin test is widely used; however, the results are often inaccurate. Positive results can be observed in patients with active tuberculosis (TB) as well as in BCG-vaccinated persons and individuals who are infected with mycobacteria but have not developed the disease. MPT64, an antigen secreted from actively growing Mycobacterium tuberculosis and ...

2015
Ting-Yu Angela Liao Alice Lau Sunil Joseph Vesa Hytönen Zakaria Hmama Anil Kumar Tyagi

Current strategies to improve the current BCG vaccine attempt to over-express genes encoding specific M. tuberculosis (Mtb) antigens and/or regulators of antigen presentation function, which indeed have the potential to reshape BCG in many ways. However, these approaches often face serious difficulties, in particular the efficiency and stability of gene expression via nucleic acid complementati...

2014
Wouter SP Jong Maria H Daleke-Schermerhorn David Vikström Corinne M ten Hagen-Jongman Karin de Punder Nicole N van der Wel Carolien E van de Sandt Guus F Rimmelzwaan Frank Follmann Else Marie Agger Peter Andersen Jan-Willem de Gier Joen Luirink

BACKGROUND The Autotransporter pathway, ubiquitous in Gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. Capitalizing on its crystal structure, we have engineered the Escherichia coli autotransporter Hemoglobin protease (Hbp) into a versatile platform for secretion and surface display of multiple heterologous proteins in one car...

Journal: :Indian journal of experimental biology 2010
Mamta Kalra Gopal Krishen Khuller Javaid Ahmad Sheikh Indu Verma

Tuberculin skin test (TST), an age old method is based on measuring delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). However, inspite of simplicity, ease and cost effectiveness, the usefulness of PPD test is limited due to its inability to distinguish among a protective immune response, latent infection and active tuberculosis disease. On the other hand, a skin...

Journal: :Indian journal of experimental biology 2011
Mohammed A A Sarhan Mustaffa Musa Zainul F Zainuddin

Expressing proteins of interest as fusion to proteins of bacterial envelope is a powerful technique for biotechnological and medical applications. The synthetic gene (VacII) encoding for T-cell epitopes of selected genes of Mycobacterium tuberculosis namely, ESAT6, MTP40, 38 kDa, and MPT64 was fused with N- terminus of Pseudomonas syringae ice nucleation protein (INP) outer membrane protein. Th...

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