The leading fungal pathogens causing systemic infections in humans are Candida spp., Aspergillus fumigatus , and Cryptococcus neoformans . major class of antifungals used to treat such the triazoles, which target cytochrome P450 lanosterol 14-α-demethylase, encoded by ERG11 (yeasts)/ cyp51A (molds) genes, catalyzing a key step ergosterol biosynthetic pathway. Triazole resistance clinical fungi ...

UNLABELLED Ergosterol is a major and specific component of the fungal plasma membrane, and thus, the cytochrome P450 enzymes (Erg proteins) that catalyze ergosterol synthesis have been selected as valuable targets of azole antifungals. However, the opportunistic pathogen Aspergillus fumigatus has developed worldwide resistance to azoles largely through mutations in the cytochrome P450 enzyme Cy...

Upc2p, a transcription factor of the zinc cluster family, is an important regulator of sterol biosynthesis and azole drug resistance in Candida albicans. To better understand Upc2p function in C. albicans, we used genomewide location profiling to identify the transcriptional targets of Upc2p in vivo. A triple hemagglutinin epitope, introduced at the C terminus of Upc2p, conferred a gain-of-func...

Candida glabrata has emerged as a common cause of fungal infection. This yeast has intrinsically low susceptibility to azole antifungals such as fluconazole, and mutation to frank azole resistance during treatment has been documented. Potential resistance mechanisms include changes in expression or sequence of ERG11 encoding the azole target. Alternatively, resistance could result from upregula...

In this study, we present a rare case of fatal breakthrough Candida tropicalis infection in a patient with acute lymphoblastic leukemia (ALL) while on posaconazole prophylaxis. Then, we explore the mechanisms underlying azole resistance by focusing on enhanced efflux pumps and changes in the azole target enzyme Erg11p, which was encoded by the ERG11 gene. Our study demonstrates that Y132C subst...

We report on the first cloning and nucleotide sequencing of an ERG11 allele from a clinical isolate of Candida kefyr cross-resistant to azole antifungals. It was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of C. kefyr. Two amino acid substitutions were identified: K151E, whose role in fluconazole resistance was already demonstra...

Limited knowledge exists on the virulence factors of Candida tropicalis and mechanisms azole resistance that lead to an intensified pathogenicity treatment failure. We aimed evaluate molecular among C. isolated from patients with candidemia. Several factors, including extracellular enzymatic activities, cell surface hydrophobicity (CSH), biofilm formation, were evaluated. Antifungal susceptibil...

ObjectivesEmergence of azole resistance may contribute to recurrences vulvovaginal candidiasis. Thus, new drugs are needed improve the therapeutic options. We studied in vitro activity ibrexafungerp and comparators against Candida albicans isolates from vaginal samples blood cultures. Furthermore, were genotyped study compartmentalization genotypes relationship between genotype antifungal susce...