An analysis of reported hepatic abundances of CYP3A4 and 3A5 indicated that values determined by immunoquantification using commercially available, unpurified recombinant enzymes as standards are significantly lower than those determined using purified enzymes or human liver microsomes characterized with lysosomal peptides (CYP3A4: mean 45 versus 121 pmol/mg protein, p < 0.01; CYP3A5: mean 28 v...

Erythrocytes are excellent models for the study of interactions of xenobiotics with biomembranes. Present work is designed to study the in vitro effects of some organophosphates (ethion, chlorpyrifos, dimethoate and monocrotophos) on rat erythrocytes. Treatment of erythrocytes with organophosphates resulted in decreased erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity, whereas ac...

Based on the presence and absence of enzyme activities, the biochemical pathways for the fermentation of inulin by Clostridium thermosuccinogenes DSM 5809 are proposed. Activities of nine enzymes (lactate dehydrogenase, phosphoenolpyruvate carboxylase, malate dehydrogenase, fumarase, fumarate reductase, phosphotransacetylase, acetate kinase, pyruvate kinase, and alcohol dehydrogenase) were meas...

plants and soil microbes are the main sources of most enzymes available in the environment. it is, therefore, anticipated that any change in the growth of soil biota in response to salinity, may reduce the synthesis and production of extra- and intra- cellular enzymes. as a result, a reduction in the growth of plants and the activity of soil microbes in saline environments may lessen the level ...

In this study, Örgü cheese has been produced by using different coagulating enzymes (calf rennet, microbial enzymes, recombinant chymosin). The effects of different coagulating enzymes which are used on the characteristic of mineral material and cheese has been observed during 90 days ripening time. Mineral material contents of Örgü cheese have been determined with ICP-OES (inductively coupled ...

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Can in silico engineering speed up the delivery of biocatalysts for the burgeoning bioeconomy? In this issue, Kamerlin and coworkers introduce CADEE [Amrein et al. (2017), IUCrJ, 4, 50-64] - a framework for Computer-Aided Directed Evolution of Enzymes - that promises to lessen the burden on 'wet lab' enzymologists when optimizing biocatalysts using laboratory-based directed evolution methods.