An enzyme-mediated universal fluorescent biosensor template for rapid detection of pathogens was developed based on the strategy a three-dimensional (3D) DNA walker and catalyzed hairpin assembly (CHA) reaction.

Vanillin was extracted from vanilla beans using pretreatment with cellulase to produce enzymatic hydrolysis, and response surface methodology (RSM) was applied to optimize the processing parameters of this extraction. The effects of heating time, enzyme quantity and temperature on enzymatic extraction of vanillin were evaluated. Extraction yield (mg/g) was used as the response value. The result...

Rewritable security printing has been successfully achieved based on a biofuel-driven transient supramolecular co-assembly mediated by an enzyme, providing fascinating potential for artificial functional materials with biomimetic mode.

Perilla seed oil riched in the ω-3 fatty acid especially α-linolenic acid, is associated with a beneficial role human health. In present study, Aqueous Enzymatic Extraction (AEE) of crude from seeds was investigated. The extraction parameters including enzyme type, solid-to-water ratio, pH, temperature, loading and hydrolysis time were determined by Response Surface Methodology (RSM). RSM resul...

In this report, we detail Substrate Mediated Enzyme Prodrug Therapy (SMEPT) as a novel approach in drug delivery which relies on enzyme-functionalized cell culture substrates to achieve a localized conversion of benign prodrug(s) into active therapeutics with subsequent delivery to adhering cells or adjacent tissues. For proof-of-concept SMEPT, we use surface adhered micro-structured physical h...

Lunasin and Bowman-Birk protease inhibitor (BBI) are two soybean peptides to which health-promoting properties have been attributed. Concentrations of these peptides were determined in skim fractions produced by enzyme-assisted aqueous extraction processing (EAEP) of extruded full-fat soybean flakes (an alternative to extracting oil from soybeans with hexane) and compared with similar extracts ...

Immunotherapy has been used for years in many types of cancer therapy. Recently, cancer immunotherapy has focused on mechanisms which can enhance the development of cell-mediated immunity. Anticancer medications are administered to inhibit immunosuppressive factors such as nagalase enzyme, which is produced by neoplastic cells and destroys macrophage activating factor (Gc-MAF). Anti-neoplastics...

In a biorefinery context it is an advantage to fractionate and extract different wood components in a relatively pure form. However, one major obstacle for efficient extraction of wood polymers (lignin, polysaccharides etc.) is the covalent lignin-polysaccharide networks present in lignified cell walls. Enzymatic catalysis might be a useful tool for a controlled degradation of these networks, t...