An enzyme-linked immunosorbent assay (ELISA) was developed for detection of staphylococcal enterotoxins in foods. The "double-antibody sandwich" protocol combines parts of several procedures reported previously. Horseradish peroxidase was conjugated to antibody specific for an enterotoxin, and the antibody-enzyme conjugate was assayed with a 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid)-H...

Introduction: There are two alternative explanations of the Stroop phenomenon. Several studies have revealed that the difference in performance on congruent and incongruent trials can arise from response interference. On the contrary, many authors have claimed that Stroop interference might occur at earlier processing stages related to semantic or conceptual encoding. The present study aims to ...

The most commonly used empirical approach to address issues of attention constraint is evaluate the degree of interference in dual or multiple tasks. The purpose of this study was designing software to estimate the relative interference between multiple tasks and determine its validity in performance prediction of simultaneous multiple tasks. in order to assess the validity of software, 46 stud...

We have studied the interference of roxithromycin with NADPH oxidase, the key enzymatic system for oxidant production by human neutrophils. Roxithromycin alters the reconstitution of an active enzyme and impairs the translocation to the outer membrane of the cytosolic components p47-phox and p67-phox. Interestingly, in resting cells roxithromycin directly triggers the translocation of these fac...

(a) HA; (b) hydrolytic product of testicular, lysosomal or venom HYASE; (c) hydrolytic product of leech HYASE; (d) hydrolytic product of bacterial HYASE. sensitivity and the production of interfering precipitates, the Morgan-Elson assay cannot be used for kinetic investigation of the enzyme. Furthermore this assay is prone to interference from compounds such as disodium cromoglycate, (+ )-catec...

followed spectrophotometrically. The spectrophotometric method could not be applied to the determination of the equilibrium constant of Reaction 1 owing to interference by lactic dehydrogenase which contaminated the best preparations of the pigeon liver enzyme available (1). However, through coupling with the isocitric dehydrogenase system (Reaction 2), Reaction 3 was obtained (3). The equilibr...

10.1128/CDLI.10.5.934-939.2003. 2003, 10(5):934. DOI: Clin. Diagn. Lab. Immunol. Sarrot-Reynauld Gaudin, Bernard Imbert, Christian Massot and Françoise Josiane Arvieux, Chantal Dumestre-Pérard, Philippe Christian Drouet, Marie-France Nissou, Denise Ponard, Antibodies and Rheumatoid Factors Minimal Interference with Antinuclear Assay Using Antigens from Cell Lysate: by an Enzyme-Linked Immunosor...

An over-the-counter Chinese medicine, Chan Su, is used as a cardiotonic agent. We demonstrated significant digoxin-like immunoreactivity in various organic and aqueous extracts of Chan Su. For example, when a 20-microL aliquot of an aqueous extract of Chan Su powder (1 mg/mL) was added to a 2-mL aliquot of a drug-free serum, the observed digoxin-like immunoreactivity was 2.76 ng/mL (3.53 nmol/L...

L-Asparaginase is immunosuppressive, inhibiting both the cellular and humoral responses, as well as the concanavalin Aand/or phytohemagglutinin-induced blastogenesis of lymphocytes. However, its mechanism of action is presently unclear. This study dealt with the enzyme effects on the lymphocyte surface leading to interference with the binding of concanavalin A. The mitogen was labeled with ' 25...