The synthesis of many chemicals needs high temperature and pressure. Many chemicals like ethanol, amino acids, citric acids, nitrates, nitrites, fine chemicals, essential compounds can be synthesized by biological pathways. Enzyme catalyzed reactions provide huge boost to the efforts concentrated on cost effective and environment friendly technology. Immobilization of enzyme has advantages such...

Immobilization is a method for making an enzyme more robust in the environment, especially terms of its stability and reusability. A mutant phosphotriesterase (YT PTE) isolated from Pseudomonas dimunita has been reported to have high proficiency hydrolyzing Sp Rp-enantiomers organophosphate chromophoric analogs therefore great potential as decontamination agent biosensor. This work aims investi...

Enzymes are capable of unique and selective transformations that can enable efficient chemical production. While many industrial processes have been developed using free enzymes in aqueous solutions, immobilizing on a solid support offer considerable advantages, including improved reaction efficiency, enzyme stability, the ability to perform reactions non-aqueous media, simplified separation pr...

The β-glucosidase from almond was immobilized using different strategies: ionic adsorption on aminated (MANAE)-agarose beads at pH 5, 7 and 9, followed by glutaraldehyde pre-activated supports. of the immobilization altered to allow enzyme molecule orientations support surface. concentration exhibited an effect stability free enzyme. Immobilization ion exchange maintained up 80% activity β-gluc...

The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold more stable than the free enzyme at 50...

BACKGROUND Immobilization of Trigonopsis variabilis D-amino acid oxidase (TvDAO) on solid support is the key to a reasonably stable performance of this enzyme in the industrial process for the conversion of cephalosporin C as well as in other biocatalytic applications. RESULTS To provide a mechanistic basis for the stabilization of the carrier-bound oxidase we analyzed the stabilizing effects...

The advantages of immobilized enzyme over its soluble counterpart arise from their improved stability andeasy separation from the reaction media, leading to decrease in production cost. Immobilization methodsrange from adsorption onto matrices, entrapment, cross-linking and covalent bonding to prefabricatedcarriers or activated supports. Changes in kinetic properties of immobi...

AaCel9A [β-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. The properties of the immobilized AaCel9A were investigated by determining the optimum pH and optimum temperature for activity, thermal stability, and kinetic parameters. The immobilization process shifted the enzyme’s optimum temperature from 65 °C for the fr...

Immobilization process is to optimize the operational performance of an enzyme for industrial applications. So far different matrices have been described in the literature to improve the performance of the immobilized enzymes. With the advent of nanotechnology, the nanomaterials because of their unique physico-chemical properties constitute novel and interesting matrices for enzyme immobilizati...