نتایج جستجو برای: enzyme assays

تعداد نتایج: 341641  

Journal: :Infection and immunity 1976
I Financsek I Ketyi W Sasak W Jankowski E Janczura T Chojnacki

Lysogenic conversion of Shigella flexneri type antigens was studied with the aid of wild-type and thermosensitive mutant phages. With all wild-type phages, the appearance of glycosylated antigen was accompanied by the appearance of polyprenyl phosphate glucose synthetase activity. With some of the mutant phages, the appearance of glycosylated antigen was not followed by the formation of lipid-l...

Journal: :Journal of Clinical Pathology 1970

Journal: :Chembiochem : a European journal of chemical biology 2004
Zhouxin Shen Eden P Go Alejandra Gamez Junefredo V Apon Valery Fokin Mike Greig Manuel Ventura John E Crowell Ola Blixt James C Paulson Raymond C Stevens M G Finn Gary Siuzdak

A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetyl...

Journal: :Prenatal diagnosis 1990
H Zhao O P Van Diggelen R Thoomes J Huijmans E Young T Mazurczak W J Kleijer

A new fluorogenic substrate, 4 methylumbelliferyl beta-D-6-sulphogalactoside, was used for the assay of galactose-6-sulphate sulphatase activity in chorionic villi, cultured villus cells, and amniocytes. The fluorometric assay is much more convenient than the conventional assay using radiolabelled, sulphated oligosaccharides. Both types of substrate were used in the prenatal diagnosis of three ...

2013
Blake-Joseph Helka John D. Brennan

Microarrays have found use in the development of high-throughput assays for new materials and discovery of small-molecule drug leads. Herein we describe a guided material screening approach to identify sol-gel based materials that are suitable for producing three-dimensional protein microarrays. The approach first identifies materials that can be printed as microarrays, narrows down the number ...

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