background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...

background: in algeria, date palm is currently confronted to the bayoud disease. biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species.objectives: callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars.materials and methods: some factors influencing the isolation ...

background : microbial monitoring of aquatic sources is essential in the correct management of these vital sources. multiple tube fermentation (mtf) technique is currently used for assessment of the microbial quality of water sources. however, today enzymatic assay has been presented as an alternative approach for detecting indicator bacteria. the purpose of this study was to compare enzymatic ...

in current study, protein hydrolysates were produced using microbial proteases of alcalase, protamex and flavourzyme from yellowfin tuna viscera. the results indicated that, protein hydrolysate from alcalase, had significantly higher protein content, nitrogen recovery and degree of hydrolysis than the others (p 0.05). the results of amino acids composition revealed that all hydrolysates were al...

esterification of chitosan with adipic acid catalyzed by immobilized candida antarctica lipase b was carried out in this study. response surface methodology (rsm) based on a four-factor- five-level small central composite design (sccd) was employed to model and analyze the reaction. a total of 21 experiments representing different combinations of the four reaction parameters including ch...

the etiopathogenesis of alzheimer's disease (ad) is still unclear.  however, long-term oxidative stress is believed to be one of the major contributing factors in progression of neuronal degeneration and decline of cognitive function in ad. in order to assess the presence of oxidative stress in ad, we examined the enzymatic activities of the erythrocyte cu-zn superoxide dismutase (cu-zn sod), g...

l-asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.in this study, simple and advantageous method has been validated for rapid and precise determination of intracellular l-asparaginasein bacterial species. a suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewa...

a long-term study was conducted between 1985 and 2003 on rehabilitation trials at sishen ironore mine, south africa, to identify grass species that would survive in the artificial growth medium appliedto the sites, and to determine the most suitable medium for sustainable vegetation growth. vegetationestablishment was tested at slopes of 18° and 34° and with five different cover materials. afte...