The effect of secondary metabolites added to cultures of the organisms producing them was investigated. Nisin was added to growing cultures of a nisin-producing strain of Streptococcus lactis (354/07) and enterotoxin B to strains of Staphylococcus aureus (S6 and 243) producing enterotoxin B. One quarter (12 mug/ml) of the amount of nisin formed by the culture of S. lactis inhibited lag-phase ce...

Background: In this study we aimed at detecting the enterotoxin A and B gene of S. aureus in clinical samples of patients attending health centers in Zahedan using molecular methods. Materials and Methods: A cross-sectional study was carried out in which 40 samples of S. aureus were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were c...

Pyrogenic responses, ranging up to 4.8 F, were induced in cats by oral administration of highly purified staphylococcal enterotoxin B in doses from 10 to 100 mug/kg. Fever was a more sensitive indicator of intoxication than was emesis. Highly purified preparations of enterotoxin A, whether administered intravenously (0.01 to 1.0 mug/kg), orally (10 to 25 mug/kg), or into the cerebral ventricles...

In contrast to Bacillus subtilis, the role of the two-component regulatory system ResDE has not yet been investigated in the facultative anaerobe Bacillus cereus. We examined the role of ResDE in the food-borne pathogen B. cereus F4430/73 by constructing resDE and resE mutants. Growth performances, glucose metabolism, and expression of hemolysin BL (Hbl) and nonhemolytic enterotoxin (Nhe) were ...

A simple homogeneous enzyme immune assay was developed for detection of staphylococcal enterotoxin B by a conjugate of beta-amylase coupled with the enterotoxin.

the aim of this study was to determine the presence of toxin genes in clostridium perfringens isolated from 200minced meat samples using molecular typing. for this purpose, after isolation of clostridium perfringens using conventional culture method and confirmation by specific 16srd na gene pcr, alpha (cpa), beta (cpb), beta 2 (cpb2),epsilon (etx), iota (ia) and enterotoxin (cpe) genes were in...

Hemolysin BL (HBL) is the multi-component enterotoxin causing diarrheal type of Bacillus cereus food poisoning. The hbl operon of B. cereus was divided into 4 coding sequences (CDS) and identified as hblC, hblD, hblA and hblB genes consisting of 1,319, 1,221, 1,128 and 1,401 nucleotides encoding proteins of 439, 406, 375 and 466 amino acids with predicted sizes of 49.3, 43.8, 41.7 and 52.3 kDa,...

BACKGROUND Accurate and rapid diagnosis of bacterial arthritis is not always possible in unvaccinated (Streptococcus pneumoniae and Haemophilus influenzae type B) children in Iran. OBJECTIVES Searching the staphylococcal superantigen (entrotoxin A, B, C and TSST1) in synovial fluid of cases with inflammatory arthritis. PATIENTS AND METHODS This cross sectional study was implemented in the p...