Polymyxin B-induced release of enterotoxin from Escherichia coli strain H-10407 was demonstrated. Incubation of E. coli cells derived from 6-h cultures with polymyxin caused the rapid release of enterotoxin with a molecular weight of approximately 20,000, as estimated by the gel filtration technique. The rapidity of the release of enterotoxin indicates that it probably resides in the periplasmi...

elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

background: in this study we aimed at detecting the enterotoxin a and b gene of s. aureus in clinical samples of patients attending health centers in zahedan using molecular methods. materials and methods: a cross-sectional study was carried out in which 40 samples of s. aureus were obtained from patients in a hospital in zahedan, iran. following the biochemical tests, identifications were conf...

Natural cholera toxoid appears to act as a competitive inhibitor of cholera enterotoxin and is thus a useful tool for studying the interaction of cholera enterotoxin with cell membranes. Cholera enterotoxin binds to gut mucosa more rapidly than does its natural toxoid. Once binding occurs, however, it appears to be prolonged for both materials. Formalinized cholera toxoid has no inhibitory effe...

Two peptide bonds of staphylococcal enterotoxin C, were hydrolyzed concurrently at quite different rates during limited digestion with trypsin. A Lys-Val at about position 92 in the disulfide loop was the first bond cleaved, followed by a Lys-Asx at about position 57 on the NH2-terminal side of the loop. Preparations of singly cleaved material (enterotoxin C1-T1) contained about 93% of the clea...

conclusions the results revealed, the specific primers that amplified the ente gene were able to amplify the staphylococcal enterotoxin-like toxin type p gene. the specific primers for the entp gene were amplified a fragmented gene (700 bp) showed 100% homology with entp reference gene and also 80% homology with enta and ente genes. results the pcr amplification method was optimized for entp ge...

BACKGROUND About 20 different types of staphylococcal enterotoxins are produced by Staphylococcus aureus, in which type A is more common in food poisoning syndrome. Also staphylococcal enterotoxin A superantigen is a potent inducer of cytotoxic T lymphocyte activity and cytokine production and could stimulate T cells containing T-cell receptor beta chain domains when binding to major histocompa...

background and objectives:   staphylococcus aureus is one of the main causatives of bovine mastitis. resistance of some strains to methicillin, can complicate the treatment of its infections. on the other hand, enterotoxin production is also im- portant. therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in s. aureus isolates caused bovine m...

since the heat-stable enterotoxin (st) produced by yersinia enterocolitica is similar to enterotoxin production by e. coli, it is probably classified among toxinogenic bacteria. the culture filtrate of st-producing strains of y. enterocolitica is able to activate guanylate cyclase and to increase fluid accumulation action of cylic guanosin 3', 5' - monophosphate (cgmp) in sucking mous...