Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

Introduction. Since 2013, European soldiers have been deployed on the European Union Training Mission (EUTM) in Mali. From the beginning, diarrhea has been among the most "urgent" concerns. Diarrhea surveillance based on deployable real-time PCR equipment was conducted between December 2013 and August 2014. Material and Methods. In total, 53 stool samples were obtained from 51 soldiers with acu...

In the present study, 470 children less than 72 months of age and presenting acute diarrhea were examined to identify associated enteropathogenic agents. Viruses were the pathogens most frequently found in stools of infants with diarrhea, including 111 cases of rotavirus (23.6% of the total diarrhea cases) and 30 cases of adenovirus (6.3%). The second group was diarrheogenic Escherichia coli (8...

We studied 2,246 episodes of childhood diarrhea over a 2-year period in a general hospital serving a population of about one million on the island of Hong Kong. Rotavirus (24%) and nontyphoid salmonellas (23%) were the most common causal agents, followed by Campylobacter sp. (9%). Rotavirus occurred largely during winter, whereas salmonellas and Campylobacter sp. occurred more commonly during s...

Abstract Enteropathogenic Escherichia coli(EPEC) and enterohemorrhagic E. coli(EHEC) are significant causative agents for diarrheal diseases globally. Vaccination has been deemed as the most effective strategy preventing EPEC EHEC caused foodborne illnesses; however, FDA not yet licensed any vaccine against or EHEC. Of note, live attenuated bacterial scarcely investigated infection, in particul...

Background and Purpose: Diarrhoeagenic E. coli (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining prevalence five main pathotypes DEC isolated from faeces children under years old with or not, living city Koula-Moutou. Methodology: Isolates were phenotypically screened on chromIDTM agar molecularly by multiplex PCR to detect pres...

Three morphologic patterns of interaction between bacteria and enterocytes have been observed in colonic biopsy specimens from AIDS patients with chronic diarrhea in the United States. The DNA encoding virulence factors and the HEp-2 cell adherence patterns of Escherichia coli strains isolated from the stools of eight symptomatic AIDS patients were compared with those of five control strains wi...

Diarrheal disease remains an unsolved problem in developing countries. The emergence of new etiological agents (non-cholera vibrios) is a major cause of concern for health planners. We attempted to unveil the seasonal dynamics of entero-pathogenic Vibrios in Gangetic riverine-estuarine ecosystem. 120 surface water samples were collected for a period of one year from 3 sampling sites on the Hoog...

Enteropathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis are both etiological agents for intestinal infection known as yersiniosis, but their epidemiology and ecology bear many differences. Swine are the only known reservoir for Y. enterocolitica 4/O:3 strains, which are the most common cause of human disease, while Y. pseudotuberculosis has been isolated from a variety of sourc...

conclusions this study suggests that epec strains are important contributors to diarrhea in iranian children. because of the weakness of routine microbiological tests and poor specifity of serological tests, it is recommended that the epec strains are better detected by molecular methods. results the e. coli isolates were confirmed by the presence of the uida gene. the epec was diagnosed in 6 c...