Background Presently, enzymes still constitute a major part of the cost of biofuel production from lignocellulosic biomass. Processive endoglucanases, which possess both endoglucanase and exoglucanase activity, have the potential to reduce the costs of biomass saccharification when used together with commercial cellulases. Therefore, the exploration of new processive endoglucanases has attracte...

Cellulolytic fungi can easily be screened within 2 d for the production of cellulolytic enzymes by staining with Congo red, or by measuring the amount of reducing sugar (glucose) produced with the dinitrosalicyclic acid reagent method. Endoglucanase activity is visible in carboxymethyl cellulose agar plates after staining with Congo red, and fixing with HCI or NaOH. This method is essentially b...

Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellu...

In this study, cellulase, endoglucanase, and ?-glucosidase activity produced by three fungal strains (Aureobasidium pullulans, Neurospora crassa Trichoderma reesei) were determined on differently processed canola substrates [hexane extracted meal (HECM), washed HECM (WHECM), seed sprout (CSM)] in mono- co-culture fermentation under solid- submerged- state processes. Results showed that the inoc...

The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The ...

It was found that, for a dissolving pulp, an enzymatic pre-treatment with an endoglucanase prior to viscose preparation did not change the correlation between the gamma number of the viscose dope and the carbon disulphide charge. Thus, the pulp stoichiometry, or reactivity, was not changed by an endoglucanase pre-treatment. It has nevertheless previously been found that the pulp reactivity, mea...

The lignocellulosic biomass (especially agricultural wastes) is known to be an excellent carbon source for microbial enzyme production. In this paper, the cellulase production from lignocellulosic materials under solid state fermentation (SSF) was investigated. The effects of fermentation conditions, such as moisture content, initial pH, temperature, and composition of mixed substrate (wheat st...

Plant expression of thermostable endoglucanase (E1) [pdb:1ECE] has been proposed for improved conversion of lignocellulose to ethanol for fuel production. Since E1 was isolated from hot springs and has thermostable properties, it may have increased stability when released to agricultural soils during post-harvest residue decomposition. Endoglucanases are a subclass of cellulases and act by clea...

Bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. One of the most important organisms that produce cellulases is the filamentous fungi, Trichoderma reesei which able to secrete large amounts of different cellulases. These enzymes are probably the most widely used cellulases industrially, however, th...