OBJECTIVES Sampling of both the cervix and urine increases the chance of detection of Chlamydia trachomatis compared with sampling either site alone. We determined the effect of combining urine and cervical swab specimens in the clinic setting on the sensitivity of C trachomatis polymerase chain reaction (PCR) testing. METHODS For each of 100 women attending a genitourinary medicine clinic wi...

OBJECTIVE The frequency of Chlamydia trachomatis in women with mucopurulent discharge was determined by a cell culture technique and a transcription-mediated amplification (TMA) assay in endocervical swab specimens. SUBJECTS AND METHODS Endocervical swab specimens were obtained from 116 symptomatic patients with genitourinary complaints or abdominal pain. All of the women were married, with a...

conclusions nucleic acid amplification tests (naats) are very appropriate in detection of infected individuals. detection techniques such as naats are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including naats with culture as gold standard for determination antimic...

A randomized cross-over trial of herpes simplex virus type 2 (HSV-2)-suppressive therapy (valacyclovir, 500 mg twice daily, or placebo for 8 weeks, a 2-week washout period, then the alternative therapy for 8 weeks) was conducted among 20 Peruvian women coinfected with HSV-2 and human immunodeficiency virus type 1 (HIV-1) who were not on antiretroviral therapy. Plasma samples (obtained weekly) a...

In an attempt to increase Chlamydiazyme (Abbott Laboratories) detection of Chlamydia trachomatis antigen and to establish the reproducibility of positive results, we carried out an investigation into the usefulness of testing duplicate specimens, of more aggressive endocervical specimen collection by using cytobrushes instead of swabs, and of the repeated testing of both specimens from patients...

Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Dia...

OBJECTIVE To investigate, by use of the Amplicor PCR in a routine setting, the recovery rate of Chlamydia trachomatis in ano-rectal and pharyngeal swab samples obtained from males and females attending an STD clinic in relation to sexual practices, symptoms, and signs. DESIGN Data regarding sexual practices, and symptoms and signs related to the rectum and pharynx, were obtained from 196 fema...

OBJECTIVES The aim of this study was to establish the prevalence of sexually transmitted infections including human immunodeficiency virus (HIV) infection in women with Bartholin's gland abscess. SETTING Gynaecology Clinic of King Edward VIII Hospital, a large urban, referral hospital for the province of Kwa-Zulu Natal, serving an underprivileged population. METHODS Thirty consecutive women...

BACKGROUND Chlamydia trachomatis is one of the major bacterial agents of the sexually transmitted diseases worldwide, especially among young females. There is no data regarding the prevalence of genital Chlamydia infection among young females in Kashan, Iran. OBJECTIVES The current study aimed to determine the prevalence of endocervical C. trachomatis infection among females aged 17 - 35 year...

Speculum examination to inspect vaginal walls, cervix, and characteristics of discharge. Although not mandatory, vaginal pH can be checked by using a swab to collect discharge from the lateral vaginal wall and rubbing on to narrow range pH paper. This can help discriminate between bacterial vaginosis, trichomoniasis, and candidiasis. Take endocervical swabs if there is risk of sexually transmit...