conclusions nucleic acid amplification tests (naats) are very appropriate in detection of infected individuals. detection techniques such as naats are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including naats with culture as gold standard for determination antimic...

an amplification polymerase chain reaction (pcr) test for the direct detection of chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. during 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specimens from ...

A coamplification PCR test for the direct detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urethral and endocervical swabs and urine samples from men and women was compared to standard culture techniques. Processed specimens were amplified in single reaction tubes containing primers for both organisms, and PCR products were detected by a colorimetric microwell plate hybridization...

AIMS To increase the sensitivity of an automated chlamydia enzyme immunoassay by significantly lowering its cut off value, and to maintain specificity by confirmation with polymerase chain reaction (PCR) and direct immunofluorescent antibody test (DFA). METHODS Over five months, the cut off value of the enzyme immunoassay used to screen urogenital samples for chlamydia antigen was reduced fro...

Aztreonam, 1 g intramuscularly, was compared with spectinomycin, 2 g intramuscularly, for uncomplicated gonorrhea. There were no failures with either drug. For aztreonam, there were 26 urethral, 3 rectal, and 3 endocervical sites that were infected. Aztreonam in a single dose of 1 g intramuscularly is satisfactory therapy for uncomplicated urethral gonorrhea in men and may be effective for rect...

This retrospective study refers to the evolution rate of cervical lesions diagnosed from cervical biopsies, respectively the endocervical scrape collected from 2001 to 2008 and found its place as an introduction to a further complex study. In this period of 8 years, in our laboratory were processed a total of 1387 biopsies and endocervical scrape. All histopathological diagnoses can be classifi...

THE PRESENCE of tissue-specific antigen in various mucins has been demonstrated in a number of studies (Bara et al., 1977; Hillermanns, 1962; Nairn et al., 1962). Antibodies to mucins from alimentary tract can be readily produced and shown by immunofluorescence to localize to endosecretory parts of the epithelial cells. More recently, Gold & Miller (1978) showed that, while common gastrointesti...