To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and an...

The improved efficacy of high avidity CTL for clearance of virus has been well-documented. Thus, elucidation of the mechanisms that confer the increased sensitivity to peptide ligand demonstrated by high avidity CTL is critical. Using CTL lines of high and low avidity generated from a TCR transgenic mouse, we have found that functional avidity can be controlled by the expression of CD8alphaalph...

ABSTRACT In this study we evaluated the performance of potato cultivars in organic production system, aiming to identify those more productive and less damaged by Phytophthora infestans Diabrotica speciosa. The experiment was conducted during 2013/2014 2014/2015 crop seasons. Cultivars Ágata, Aracy Ruiva, Vitória, Clara, Eliza, Catucha Cris were assessed for severity area under disease progress...

These and other letters, including a letter from Dr L. I. Taft reporting on the first brain the Walter and Eliza Hall Institute (WEHI) received, are not included in the published Gajdusek correspondence of the early days. On 4th February, John Gunther sent another letter to Gray Anderson in which he wrote, ‘The Government Anthropologist has visited Fore and informs me the Administration is losi...

This study evaluates whether tip of the tongue experiences (TOTs) are caused by a more accessible word which blocks retrieval of the target word, especially for older adults. In a "competitor priming" paradigm, young and older adults produced the name of a famous character (e.g., Eliza Doolittle) in response to a question and subsequently named a picture of a famous actor or actress depicting t...

In this study, we analysed IgG avidity to p 15.5 kD, p 17 kD and p 47 kD antigens by a new avidity assay termed avidity western blot. The determination was performed in two groups. Group I consisted of 27 patients with primary and secondary syphilis and group II comprised 10 subjects with latent syphilis. We found that low avidity antibodies were directed against the p 17 kD and p 47 kD antigen...

BACKGROUND Accurate and practical biologic tools to estimate HIV incidence is crucial to better monitor the epidemic and evaluate the effectiveness of HIV prevention and treatment programs. METHODS We evaluated two avidity assays to measure recent HIV infection: the Sedia HIV-1 LAg-Avidity EIA (Sedia Biosciences, Portland) and the Centers for Disease Control and Prevention (CDC)-modified Bio-...

High avidity cytotoxic T lymphocytes (CTL) are important in viral clearance and anti-tumor immunity, however, mechanisms for their optimal generation and maintenance in vivo remain unclear. Immunizing mice with an antibody-DNA vaccine encoding a single CTL epitope, induces a 100 fold higher avidity response than peptide vaccination with the identical epitope. The high avidity response is retain...