9 Abstract—The paper describes a protocol to fabricate cell10 laden microgel assemblies with pre-defined micro-architec11 ture and complexity by a bottom-up approach, which can be 12 used for tissue engineering applications. The assembly 13 process was driven by hydrophobic effect in the water/oil 14 interface. By agitating hydrophilic microgels in hydrophobic 15 medium, the shape-controlled mi...

In many emerging high value electrospinning applications, the diameter distribution of electrospun nanofibers has important implications for the product’s performance and process economics. However, the current state-of-the-art electrospinning process results in varying diameter distributions, both during a run and from run-to-run. To address this problem, our research is focused on developing ...

Electrospinning or electrostatic fiber spinning is an emerging technology that requires electric fields for the formation of non-woven materials that can be used in clothing, highperformance filters, separation membranes, scaffolds in tissue engineering, reinforcement in composite materials, templates for the preparation of functional nanotubes, and many others applications [1]. Polycaprolacton...

Nanofibrillar forms of proteins were initially recognized in the context of pathology, but more recently have been discovered in a range of functional roles in nature, including as active catalytic scaffolds and bacterial coatings. Here we show that protein nanofibrils can be used to form the basis of monodisperse microgels and gel shells composed of naturally occurring proteins. We explore the...

The electrospinning of gelatin with tunable fiber morphology from round to flat/ribbon was shown, and the detailed studies were conducted to correlate the fiber morphology with electrospinning process parameters and gelatin concentration in electrospinning solution. Particularly, variations in the applied voltage and the concentration of gelatin led to the transition of fiber shape from round t...

Microfabrication technology provides a highly versatile platform for engineering hydrogels used in biomedical applications with high-resolution control and injectability. Herein, we present a strategy of microfluidics-assisted fabrication photo-cross-linkable gelatin microgels, coupled with providing protective silica hydrogel layer on the microgel surface to ultimately generate gelatin-silica ...

Amphiphilic microgels of different composition based on the hydrophilic, pH-responsive acrylic acid (AA) and the hydrophobic, non-ionic n-butyl acrylate (BuA) were synthesised using a lab-on-a-chip device. Hydrophobic droplets were generated via a microfluidic platform that contained a protected form of AA, BuA, the hydrophobic crosslinker, ethylene glycol dimethacrylate (EGDMA), and a free rad...

The effect of PEGylation on the thermal response and protein adsorption resistance of crosslinked PNIPAm microgels was investigated. It was found that the presence of PEG, its molecular weight (M(n) 300 and 1100 g/mol) and its concentration (10, 20, and 30 wt.%) each significantly influenced both the value and breadth of the volume phase transition temperature (VPTT) and the adsorption of bovin...

Amphiphilic microgels based on the hydrophilic acrylic acid (AA) and hydrophobic crosslinks of different compositions were synthesised using a lab-on-a-chip device. The microgels were formed by polymerising hydrophobic droplets. The droplets were generated via a microfluidic platform and contained a protected form of AA, a hydrophobic crosslinker (ethylene glycol dimethacrylate, EGDMA) and a fr...