The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of ...

The production and assembling of disposable electrochemical AFM1 immunosensors, which can combine the high selectivity of immunoanalysis with the ease of the electrochemical probes, has been carried out. Firstly immunoassay parameters such as amounts of antibody and labelled antigen, buffer and pH, length of time and temperature of each steps (precoating, coating, binding and competition steps)...

This paper describes an electrochemical enzyme-linked immunosorbent assay (ELISA) on paper-based microfluidic devices. This paper-based immunoassay method has several advantages over conventional ELISA, such as short assay time, low cost, and high sensitivity. The limit of detection (LOD) of rabbit IgG used as a model system is ~ 3.9 fM; this value is lower than the LOD of paper-based colorimet...

We describe a new class of impedance-based lab-on-chip immunosensors in which the immunoagglutination of latex is monitored by electrochemical impedance spectroscopy (EIS). Antibody-coated latex microspheres agglutinated in the presence of target antigens are precipitated from solution between interdigitated microsized electrodes on a chip. Impedance spectra are reproducibly measured in the 0.1...

We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in...

A high-throughput, simple, fast, low-cost and sensitive paper-based electrochemical immunodevice has been demonstrated based on a functionalized 3D paper-based device for point-of-care diagnosis.

An electrochemical DNA biogate was designed for highly sensitive homogeneous electrochemical immunoassay by combining target-induced proximity hybridization with a mesoporous silica nanoprobe (MSN). The electroactive methylene blue (MB) was sealed in the inner pores of MSN with single-stranded DNA. In the presence of target protein and two DNA-labeled antibodies, the formed proximate complex co...