We report a rapid, ultrasensitive, immobilization-free and signal-on electrochemical DNA biosensor with excellent discrimination ability for single-nucleotide polymorphisms. It achieves recognition signal generation by the specific cleavage function of exonuclease III and the diffusion different between oligonucleotides and mononucleotides towards a negatively charged ITO electrode surface. Mea...

The focus of this work was on designing a label-free DNA biosensor based on a super-sandwich assay using the electrochemical impedance spectroscopy technique. For this purpose, we designed a signal-up configuration whose linker probes could hybridize with two regions of the target DNA. In this configuration, the presented target DNA would effectively decrease the electron transfer, which would ...

To overcome early cancer detection challenges, diagnostic tools enabling more sensitive, rapid, and noninvasive are necessary. An attractive target for blood tests is human Ecto-NOX disulfide–thiol exchanger 2 (ENOX2), expressed in most types regularly shed into sera. Here, we developed an electrochemical DNA-based (E-DNA) biosensor that rapidly detects physiologically relevant levels of ENOX2....

A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...

Genetically modified organisms (GMOs) are plants or animals whose genetic make-up has been transformed by recombinant DNA technology, which has new features such as resistance to herbicides, virus and insect. Recently, genetic modification of food products has increased in order to reduce world poverty and hunger and increase food production However, the impact of GMOs on the human health is a ...

Paper-based microfluidic devices have emerged as simple yet powerful platforms for performing low-cost analytical tests. This paper reports a microfluidic paper-based electrochemical biosensor array for multiplexed detection of physiologically relevant metabolic biomarkers. Different from existing paper-based electrochemical devices, our device includes an array of eight electrochemical sensors...