نتایج جستجو برای: ejaculated semen
تعداد نتایج: 14662 فیلتر نتایج به سال:
Objective: To develop methods for the isolation and purification of round spermatids (RS) from ejaculated semen specimens recovered from patients suffering from nonobstructive azoospermia for the purpose of performing intracytoplasmic round spermatid injections (ROSI) into human oocytes. Design: Prospective study. Setting: Audrology Institute of Lexington, Lexington, Kentucky. Patients: Six men...
OBJECTIVE We investigated the activation of caspases 8, 9, 1, and 3 in human ejaculated spermatozoa to study main pathways of apoptosis. Potential functional impact of this phenomenon and possible activation mechanisms were examined by [1] subjecting cells to freezing and thawing, and [2] testing the dependence of caspase activity on membrane integrity. DESIGN Experimental study. SETTING An...
Repetitive electroejaculation is probably extremely stressful in conscious animals and could adversely affect fertility. The present study was designed to (a) evaluate the effects of anesthesia (40 mg ketamine/kg body weight, i.m.) on a method of electroejaculation used previously in conscious chinchillas (Chinchilla lanigera), and (b) determine the quality of the electroejaculated semen obtain...
This commentary is to critique the revised World Health Organization (WHO) semen analysis manual as it pertains to characteristics of a spermatozoon at spermiation. The aims of the revised WHO manual include improving the 'quality of semen analysis' without any restriction to clinical use. Furthermore, the manual states that semen analysis may be useful for (a) 'investigating male fertility sta...
OBJECTIVE To evaluate sperm concentration, morphology and motility of Brazilian semen donors from 1992 to 2003, in the city of São Paulo. METHODS Retrospective study analyzing 182 donor semen samples from 1992 to 2003. The first and the second donated sample were analyzed for each donor. Donor average age was 30.8 years. Means with standard errors, medians with minimum and maximum values, and...
OBJECTIVE To evaluate effects of cellular phone radiofrequency electromagnetic waves (RF-EMW) during talk mode on unprocessed (neat) ejaculated human semen. DESIGN Prospective pilot study. SETTING Center for reproductive medicine laboratory in tertiary hospital setting. SAMPLES Neat semen samples from normal healthy donors (n = 23) and infertile patients (n = 9). INTERVENTION(S) After l...
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejac...
The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing local ram semen with different combination BSA concentration. object was ten ejaculated ram, three years old. design used CRD (completely randomize design) four treatments concentration on upper and bottom layer (T1: 3% & 6%, T2: 4% T3: 5% 10%, T4: 6% 12%), 10 repetitions. Data analysis using variance Dunca...
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