To investigate the structure-function relationship of human apolipoprotein A-IV (apoA-IV), several deletion mutants of this protein were constructed by sequentially removing pairs of 22-residue repeats, potentially having an amphipathic alpha-helical conformation. The mutants, produced as recombinant poly-histidine-tagged apolipoproteins (t-apo) in Escherichia coli, assembled with phosphatidylc...

Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s. A variety of observations indicate that osmosis induces a parallel lipid flow between the...

The lecithin which is used in cardiolipin antigens for the sero-diagnosis of syphilis is extracted from beef heart tissue or egg yolk by the methods of Pangborn, Maltaner, Tompkins, Beecher, Thompson, and Flynn (1951). The product is actually a mixture of individual lecithins containing chiefly unsaturated but also some saturated fatty acids, the composition varying with the source and method o...

Silica gel thin-layer chromatography was employed to detect lecithinase activity induced from bacterial resting cell preparations induced from bacterial resting cell preparations incubated at 37 C for 4 h in the presence of purified egg yolk lecithin. Bacillus subtilis, Bacillus cereus, Serratia marcescens, and Pseudomonas aeruginosa hydrolyzed lecithin with the formation of free fatty acids as...

fhere have been many works on lecithin contained in the brain of human and mammal living on land as well as on lecithin in the egg of hen. The works on lecithin in the brain of human and mammal were reported by Yokoyama & Suzuki (1932 ab) md Levene & Rolf (1921, 1922). Reviewing the works on lecithin in the brain )f sea mammal, Igarashi & Zama (1955) studied on lecithin in the brain of sperm wh...

the aim of this research was the collection and freezing of honey bee sperm, apis mellifera, as a trail and evaluating different semen extenders to improve sperm quality after freeze-thawing process as a first trail in iran. semen was collected using artificial insemination device. in this experiment three different extenders including; egg yolk (tey), buffer and 0.5% soybean lecithin (tsl0.5) ...

Using complementary physical-chemical techniques we defined five different crystallization pathways as functions of time (30 days) and increasing lecithin (egg yolk) content in pathophysiologically relevant model biles super-saturated (cholesterol saturation indices, 1.2 - 2.7) by dilution of approximately equal to 29 g/dl bile salt-lecithin-cholesterol micellar solutions. As evidenced by quasi...

Lecithin can be prepared on a relatively large scale, free of colored impurities, by a simple two-column procedure. Commercial crude egg lecithin is partially purified by a single-step passage through an alumina column. It is then purified by a two-step passage through a prepacked, commercial silica gel column. The lecithin is prepared in solvent-free form for weighing by lyophilization from cy...

This investigation demonstrated that antigenic expression in liposomal model membranes may be markedly influenced by phospholipid composition. Incorporation of dinitrophenylaminocaproylphosphatidylethanolamine (Dnp-Cap-PE) into egg lecithin/cholesterol/dicetylphosphate bilayers did not significantly enhance the response of AKR mice to this synthetic amphipathic antigen. In contrast, the immunog...