The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and no...

The RapidChek SELECT Salmonella Enteritidis Test System was validated for the detection of Salmonella Enteritidis (SE) in poultry house drag swabs, shell egg pools, and chicken carcass rinsates. The method utilizes RapidChek SELECT Salmonella (AOAC PTM License No. 080601) proprietary primary and secondary enrichment media. Following enrichment, an immunochromatographic test strip is inserted in...

This study was designed to detemine if conjugated linoleic acid (CM) is incorporated into egg lipids through dietary CLA supplernentation. Forty Single Comb White Leghorn layen (28 weeks of age) were randomly assigned into four treatments of varying CLA levels, 0, 0.01, 0.5 and l g C W k g diet. C U replaced an equivalent arnount of linoleic acid in the diet. Eggs were collected over 36 days. F...

As a pretreatment technique to trace analysis, polyoxyethylene lauryl ether (POELE10)salt aqueous two-phase extraction system (ATPES) is coupled with high performance liquid chromatography to analyse thiamphenicol (TAP) in egg, milk and honey. The analysis of the phase behaviors of the ATPES found that the extraction efficiency and enrichment factor of TAP was influenced by the types of salts, ...

BACKGROUND Evidence showed that the occurrence of iodine deficiency endemic areas has been found in every provinces of Thailand. Thus, a new pilot programme for elimination of iodine deficiency endemic areas at the community level was designed in 2008 by integrating the concept of Sufficient Economic life style with the iodine biofortification of nutrients for community consumption. METHODS A...

Specific digestion of proteins is an essential step for mass spectrometry-based proteomics, and the chemical labeling of the resulting peptides is often used for peptide enrichment or the introduction of desirable tags. Electrochemical oxidation yielding specific cleavage C-terminal to tyrosine (Tyr) and tryptophan (Trp) residues provides a potential alternative to enzymatic digestion and a pos...

We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five rounds of selection. The final aptamer had a dissociation constant of 2.8 +/- 0.3 nM, 6.1 +/- 0.5 n...

At 56 weeks of age, 30 Hyline (W-377) laying pullets were randomly distributed in individual (1 pullet per cage) 45 x 45 x 35 cm cages. Cracked egg was used as protein supplement to the diet. Three groups of 10 Hyline laying pullets each were formed. Three isocaloric and isonitrogenous diets were prepared from different protein sources as followed: ration 1 (control) − soybean meal; ration 2 (e...