OBJECTIVE The aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. In addition, we aimed to evalu- ate the effect of lactobacilli on the expression of human papilloma virus (HPV) onco- genes. MATERIALS AND METHODS In this experimental study, using quantitative real-time polymer- ase chain reaction (PCR), we analyzed the e...

ing that a small noncanonical RING finger protein, Rbx1 (also referred to as ROC1 and HRT1), is an essential component of SCF (Skp1/cullin-1/F-box protein) E3 complexes and that a complex containing Rbx1 and Until recently no specific function had been ascribed to Cdc53/cullin-1 (CUL-1) is sufficient to mediate ubiqui-the RING finger beyond a role in dimerization of several tination in vitro. R...

results in 12 patients (37%), human papilloma virus was detected by pcr method. in addition, there was no difference in distribution of patients with respect to affliction with human papilloma virus between high-risk and low-risk groups. there was no relationship between clinical manifestation and type of papilloma virus. materials and methods this cross-sectional study was conducted on 32 pati...

background warts are benign tumors of the skin and mucosa and are caused by human papilloma virus (hpv). they are one of the most common skin diseases found mainly in children and adolescents. although most of the warts disappear spontaneously or respond to the usual therapeutic measures, resistant forms of the warts impose a problem in dermatology. intralesional injection of bleomycin has been...

background: in 1970, human papillomavirus (hpv) was introduced as the main etiologic factor of cervical carcinoma. since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as pcr have particular importance in accurate, early and definite diagnosis of the virus. so, in this research, our goal is to use a propriet...

nantly target HIV proteases 1 and 2, it is also known that part of their efficacy is due to selective inhibition of the proteasome. The pathogenicity of high-risk human papilloma virus (HPV) is dependent on expression of viral E6 proteins which inappropriately activate the 26S proteasome to degrade p53 and other cellular proteins that are detrimental to viral replication. Comparison of the abil...

High-risk human papilloma virus (HPV) is the leading cause of cervical cancer. HPV oncogenes are responsible for the development of malignancy, and the E6 oncoprotein that HPV expresses induces the degradation of tumour suppressor protein p53 (p53). This degradation leads to the upregulation of p16; however, unidentified proteins may also serve a role in the development and progression of cervi...

A tissue-specific promoter can control downstream gene expression in tissues or organs. The human involucrin (hINV) promoter (pINV) that contains 2474 bp of hINV upstream sequence is able to regulate tissue-specific gene expression. This tissue specificity may be important for the prevention and treatment of human papilloma virus infections. pINV was cloned by polymerase chain reaction and the ...

The thymidine analogue 4-thiothymidine (S(4)TdR) is a photosensitizer for UVA radiation. The UV absorbance spectrum of S(4)TdR and its incorporation into DNA suggests that it might act synergistically with nonlethal doses of UVA to selectively kill hyperproliferative or cancerous skin cells. We show here that nontoxic concentrations of S(4)TdR combine with nonlethal doses of UVA to kill prolife...

PURPOSE To establish the immortalized human corneal endothelial cell line (IHCEn) by transducing human papilloma virus (HPV) 16 E6/E7 oncogenes, and to identify their characteristics when cultivated on a lyophilized human amniotic membrane (LAM). METHODS Primary human corneal endothelial cells (PHCEn) were infected using a retroviral vector with HPV 16 E6/E7, and transformed cells were clonal...