Classically, isolation of CSCs from tumors exploits the detection of cell surface markers associated with normal stem cells. Invariable expression of these cell surface markers in almost all proliferating tumor cells that albeit impart specific functionality, the universality, and clinical credibility of CSC phenotype based on markers is still dubious. Side Population (SP) cells, as defined by ...

Fluorescence imaging of cells and subcellular compartments is an essential tool to investigate biological processes and to evaluate the development and progression of diseases. In particular, protein-protein interactions can be monitored by Förster resonance energy transfer (FRET) between two proximal fluorophores that are attached to specific recognition biomolecules such as antibodies. We inv...

Moving in line with the postulation that metadiscourse (MD) markers help transform a dry and tortuous piece of text into a coherent and reader-friendly one, the researchers in the current study attempted to investigate the effect different metadiscourse markers might have on Iranian EFL learners’ perception of written texts. To this end, 120 undergraduate English students were given three diffe...

background & aims of the study: dyes are one of the widespread chemicals which may have many adverse effects on life cycle. thus, this study was conducted to survey the rb19 dye removal efficacy from aqueous solution, using the sio2 nanoparticles. materials and methods: this study was an experimental-lab study. the batch adsorption system were utilized to evaluated the rb19 dye removal efficien...

Metadiscourse is a widely used term in current discourse analysis and language education, referring to an interesting, and relatively new approach to conceptualizing interaction between text producers and their texts and between text producers and users. Despite the growing importance of the term, however, it is often understood in different ways and used to refer to different aspects of langua...

abstract background and purpose:the main objectives of this research were to evaluating the application of electrocoagulation process for 198 dye from the aqueous phase and determining the optimum operating conditions to the dye removal using aluminum and iron electrodes. materials and methods:the present study was conducted in bench-scale. the spectrophotometer dr 5000 was used to determine th...

DNA marker plays important role as valuable tools to increase crop productivity by finding plausible answers to genetic variations and linking the Quantitative Trait Loci (QTL) of beneficial trait. Prior approaches in development of Short Tandem Repeats (STR) markers were time consuming and inefficient. Recent methods invoking the development of STR markers using whole genomic or transcriptomic...

Although many cancers are maintained by tumor-initiating cells, this has not been shown for mesenchymal tumors, in part due to the lack of unique surface markers that identify mesenchymal progenitors. An alternative technique to isolate stem-like cells is to isolate side population (SP) cells based on efflux of Hoechst 33342 dye. We examined 29 mesenchymal tumors ranging from benign to high-gra...

Neurite outgrowth is an important morphological phenotype of neuronal cells that correlates with their function and cell health, yet there are limited methods available for measuring this phenomenon. Current approaches to measuring neurite outgrowth are laborious and time-consuming, relying largely upon immunocytochemical staining of neuronal markers (e.g., beta-III tubulin or MAP2) followed by...

Repeated amphetamine administration is known to produce changes in corticoaccumbens function that persist beyond termination of drug administration. We have found previously that long-term alteration in dopamine systems leads to changes in gap junction communication, expressed as dye coupling, between striatal neurons. In this study, the cellular bases of amphetamine-induced changes were examin...