Most WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work analyzes the effects of VvWRKY1 overexpression in grapevine. Microarray analysis showed that genes e...

UNLABELLED PREMISE OF THE STUDY Demand for fresh-market sweet basil continues to increase, but in 2009 a new pathogen emerged, threatening commercial field/greenhouse production and leading to high crop losses. This study describes a simple and effective staining method for rapid microscopic detection of basil downy mildew (Peronospora belbahrii) from leaves of basil (Ocimum basilicum). • ...

Plant disease resistance is commonly triggered by early pathogen recognition and activation of immunity. An alternative form of resistance is mediated by recessive downy mildew resistant 1 (dmr1) alleles in Arabidopsis thaliana. Map-based cloning revealed that DMR1 encodes homoserine kinase (HSK). Six independent dmr1 mutants each carry a different amino acid substitution in the HSK protein. Am...

PREMISE OF THE STUDY Simple sequence repeat (SSR) markers were developed for Plasmopara obducens, the causal agent of the newly emergent downy mildew disease of Impatiens walleriana. METHODS AND RESULTS A 202-Mb draft genome assembly was generated from P. obducens using Illumina technology and mined to identify 13,483 SSR motifs. Primers were synthesized for 62 marker candidates, of which 37 ...

Many cultivars of lettuce (Lactuca sativa L.), the most popular leafy vegetable, are susceptible to downy mildew disease caused by Bremia lactucae. Cultivars Iceberg and Grand Rapids that were released in the 18th and 19th centuries, respectively, have high levels of quantitative resistance to downy mildew. We developed a population of recombinant inbred lines (RILs) originating from a cross be...

in survey of infected samples to downy mildew of broadbean, chenopodium murale and rumex chalpensis using olympus microscope (bh2) and valid keys and recently published papers, two species were identified (choi et al. 2009, ershad 2009, telle & thines 2012).

Field surveys were conducted in 3 major grapevine growing districts in Tamil Nadu viz., Coimbatore, Theni and Dindugal. The downy mildew infection on leaves was the highest (PDI 76.92 in Cumbum of Theni district. In the survey on the incidence of powdery mildew showed that the highest incidence of PDI on leaves, inflorescence and fruits in Cumbum of Theni with records of 65.49, 63.56, 61.23 per...

background: downy mildew caused by plasmopara halstedii is one of the most devastating diseases of sunflower worldwide. so far several dominant resistance genes designated as pl have been identified and their molecular markers linked to these genes are available. however, no information on the resistance genes is available in iranian lines. objective: in this study, the presence of three map-ba...

Plasmopara viticola is a biotrophic pathogenic oomycete responsible for grapevine downy mildew. We present here the first draft of the P. viticola genome. Analysis of this sequence will help in understanding plant-pathogen interactions in oomycetes, especially pathogen host specialization and adaptation to host resistance.