A highly sensitive adenylate cyclase assay method has been developed which employs sequential chromatography on columns of Dowex cation exchange resin and aluminum oxide. With the use of [a-“‘PIATP as substrate, this method permits the nearly complete separation of cyclic [“‘PIAMP formed from the substrate and other “P-containing compounds, i.e., “P in the assay blanks was barely detectable. In...

While experiential qualities of rectangular architectural spaces can be effectively predicted from their proportions and area (Franz, von der Heyde, & Bülthoff, 2003), these factors obviously cannot be directly transferred on open-plan indoor spaces. We introduce an approach that relates experiential qualities of arbitrarily shaped architectural spaces to their spatial form using isovists (Bene...

A fraction enriched in the minor components of the kanamycin complex was prepared from a commercial sample. The enriched fraction was subjected to Amberlite CG-50 and Dowex 1-X2 column chromatography. Two minor components were isolated in addition to the known A, B, and C components. These congeners were identified as paromamine and 6-o-(3-amino-3-deoxy-alpha-d-glucopyranosyl)- deoxystreptamine...

Thirty peptides obtained from the tryptic digestion of oxidized staphylococcal enterotoxin B were isolated chromatographically on columns of Dowex SOW-X2 and AGl-X2 by using pyridine-acetic acid and N-ethylmorpholine-pyridine-acetic acid buffer systems. The isolated peptides were further purified by paper chromatography and electrophoresis. The amino acid composition and sequence of each purifi...

The article considers a sorption method for extracting copper ions from model nitric acid solution based on of electrolytic refining silver with using sorbents bis-picolylamine functional groups: Dowex M-4195 and AXIONIT BPA. possibility to residual concentrations in the less than 10 mg/l is shown. working capacity both samples under batch conditions, as well dynamic exchange full ?onditions (c...

Oxidized and reduced carboxymethylated enterotoxin B was digested with chymotrypsin. The peptides were fractionated on AGl-X2 and Dowex 5OW-X2, and further purified by paper chromatography and electrophoresis. A total of 67 pure peptides was isolated and their sequences were determined completely or partially. These peptides provide important overlaps of tryptic peptides previously isolated and...

A purified compound of the activator of glucuronide formation was isolated from a boiled extract of rat liver by charcoal adsorption, ethanol fractionation of barium salts, and finally paper and Dowex-l column chromatographies. The analytical data and the chemical properties of the compound sugggested that the endogenous activator of glucuronide formation in rat liver might be uridine diphospha...