The glycosaminoglycans of isolated rabbit corneal stroma, clamped between two lucite plates at near normal hydration, were digested with testicular hyaluronidase in saline solution. After equilibration with 0.9 per cent saline solution alone the sodium and chloride content of the stroma was determined. Chloride was in equilibrium with both normal and hyaluronidase-treated stroma, allowing use o...

It has been shown, within the probable limit of error of the methods of measurement employed, that the Donnan equilibrium determines the distribution of H and Cl ions between the cell and the surrounding fluid. This equilibrium is a consequence of the impermeability of the cell membrane to the inorganic cations of the cell. The mechanism responsible for this equilibrium is suggested as that con...

Membrane potential of native isolated pepper cuticles were measured with NaCl and CaCl2 solutions for a wide range of concentrations (10BC(M)B10). From membrane potential results the fixed charge concentration, X, and the transport number of the ions in the cuticular membrane were obtained. Taking into account the X value, an estimation of the Donnan potential contribution to the membrane poten...

The effects of ionic strength and solvent polarity on the equilibrium distribution of fluorescein (FL) and FITC-dextran between the interior of polyelectrolyte multilayer microcapsules filled with negatively charged strong polyelectrolyte and the bulk solution were systematically investigated. A negatively charged strong polyelectrolyte, poly(styrene sulfonate) (PSS), used for CaCO3 core fabric...

Electrode potential measurements have been applied to study electrical characteristics like transport numbers, permselectivity & fixed charge density of ion-exchange membranes. Membrane potential measurements were made across zirconium phosphotungstate cation-exchange membrane maintained at 27◦C, using halide salts of alkali & alkaline earth metals as electrolytes. The effect of concentration o...

A system consisting of two aqueous solutions, containing equal concentrations of lactic acid, but different concentrations of Na lactate, separated by a layer of amyl alcohol has been described. This system exhibits electrical properties ranging (as the concentration of NaL is increased) from those characteristic of a simple Donnan equilibrium to those characteristic of simple diffusion. The fa...