نتایج جستجو برای: dna release
تعداد نتایج: 711962 فیلتر نتایج به سال:
Sensitive detection of miRNA-21 provides remarkable results for the diagnosis early breast cancer. Here, we fabricated a novel electrochemical biosensor ultrasensitive via in situ reduction gold nanoparticles (AuNPs)-decorated Ti 3 C 2 MXene, combined with cascaded signal amplification strategy, that is, strand displacement DNA walker-induced multi-DNA (product DNA) release and cyclic enzymatic...
Extracellular DNA (eDNA) is produced by several bacterial species and appears to contribute to biofilm development and cell-cell adhesion. We present data showing that the oral commensals Streptococcus sanguinis and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H(2)O(2)). Surprisingly, S. sanguinis and S. gordonii cell int...
Viral DNA injection into host cells is one of the primary mechanisms of viral propagation. Drug development that targets viral propagation requires fast and sensitive methods for monitoring the release of viral DNA in vitro. Here we demonstrate the use of capillary electrophoresis (CE) for monitoring DNA release from virus particles. As a model for this study, we used T5 bacteriophages that inf...
The controlled delivery of low-molecular weight drugs and proteins from biodegradable polymers has received considerable attention. However, controlled release studies of pDNA from such polymers have not been reported to date. In this study, a plasmid DNA was complexed with the cationic polymer called polyethylenimine (PEI). This gene vector has been shown to be very effective in transfecting c...
DNA and cetyltrimethylammonium bromide (CTAB) have been used to prepare gel particles for controlled release studies. This article reports on the release of DNA and CTAB in four different solutions: in sodium bromide, in strong acid, pH 2 and pH 9 solutions for salmon testes DNA-CTAB gel particles. Also, compares results at extreme acid media and 10 mM NaBr solution with higher molecular weight...
Purpose: Chitosan has a high potential for transferring DNA molecules into mammalian cells because of its cationic properties. In the present study, we have investigated DNA encapsulation efficiency and loading capacity of chitosan microparticles prepared in different conditions, as well as in vitro DNA release from microparticles, and transfection of different cell lines with chitosan-DNA micr...
Highly porous poly(D,L-lactic-co-glycolic acid) (PLGA) scaffolds were fabricated by a thermally-induced phase-separation (TIPS) method to deliver plasmid DNA in a controlled manner. A variety of TIPS parameters directly affecting pore structures and their interconnectivities of the scaffold, such as polymer concentration, solvent/non-solvent ratio, quenching methods and annealing time, were sys...
We have previously shown that RNA polymerase II (Pol II) pause release and transcriptional elongation involve phosphorylation of the factor TRIM28 by the DNA damage response (DDR) kinases ATM and DNA-PK. Here we report a significant role for DNA breaks and DDR signalling in the mechanisms of transcriptional elongation in stimulus-inducible genes in humans. Our data show the enrichment of TRIM28...
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