DNA isolation from blood is a commonly used application to obtain nDNA and mtDNA. It was previously shown that could be performed the pellet obtained after centrifugation of freeze-thawed (FTB), this pretreatment had constructive results on isolation. However, which levels can for pretreatment, their effects are unknown. The aim study determine appropriate show isolated DNA. For purpose, isolat...

BACKGROUND Circulating cell-free DNA (ccf-DNA) is becoming an important biomarker for cancer diagnostics and therapy monitoring. The isolation of ccf-DNA from plasma as a "liquid biopsy" may begin to replace more invasive tissue biopsies for the detection and analysis of cancer-related mutations. Conventional methods for the isolation of ccf-DNA from plasma are costly, time-consuming, and compl...

AIM To provide a more efficient method for isolating DNA from peripheral blood for use in diagnostic DNA mutation analysis. METHODS The use of blood impregnated filter paper and Chelex-100 in DNA isolation was evaluated and compared with standard DNA isolation techniques. RESULTS In polymerase chain reaction (PCR) based assays of five point mutations, identical results were obtained with DN...

In DNA methylation, methyl groups are covalently bound to CpG dinucleotides. However, the assumption that methyl groups are not lost during routine DNA extraction has not been empirically tested. To avoid nonbiological associations in DNA methylation studies, it is essential to account for potential batch effect bias in the assessment of this epigenetic mechanism. Our purpose was to determine i...

Isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue remains a laborious task for clinical laboratories and researchers who need to screen several samples for genetic variants. The objective of this study was to evaluate DNA isolation methods from FFPE tissues and to choose an efficient method with less hands-on time to obtain DNA of optimum concentration and purity for use in ...

A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon ...

Isolation of bacteriophage λ DNA is among the most common procedures used by those working with recombinant DNA methodologies. Although there are many protocols for λ DNA isolation (1,2,4), some are cumbersome and time-consuming, and others result in poor DNA yield. Among the most important parameters for successful phage DNA isolation is estimating the correct phage:host cell ratio for infecti...

DNA, the well-known molecule that carries genetic information of almost all forms life, represents a pivotal element in formulating intelligent and versatile micro/nanorobotic systems. DNA-functionalized micro/nanorobots have opened new exciting opportunities many research areas due to synergistic combination self-propulsion at micro/nanoscale high specificity programmability DNA interactions. ...

There are several biological sources which could be used for DNA isolation and genetic testing with potential application in the clinical setting [1]. Increasingly, DNA isolated from buccal swabs and, more recently, saliva samples is under investigation for potential use in the clinical setting rather than traditional blood samples due to ease of access, storage, and transport, as well as relat...