Preparation of Chromatin and Mononucleosome from Rat Liver Purified nuclei were digested with micrococcal nuclease for the preparation of chromatin. Prolonged digestion of the nuclei with micrococcal nuclease was used to prepare mononucleosome with a DNA length of 146 base pairs. Selective solubilization of mononucleosome was done by the acidification (NaH2PO4 1.78% w/v) of the supernatant. The...

Preservation of bio-specimens for molecular biological applications traditionally involves freezing which increases laboratory and research project costs. Protection and stabilization of DNA at room temperature (RT) may eliminate the costs associated with freezer storage. However, there is a paucity of information describing the yield, purity, and integrity of DNA stored at RT. Objectives were ...

Successful DNA amplification is vital for the detection of specific DNA targets in feeds, and this in return depends on the ability of DNA extraction methods to produce good quality DNA. In this study, seven methods were compared for DNA extraction from feeds using quantitative polymerase chain reaction (PCR) of single copy maize (Zea mays) endogenous hmg (high mobility group) gene. Relative le...

DNA extraction is the first step in obtaining high-concentration and high-purity that can use subsequent steps. Because leaf structure challenging contains several secondary metabolites affect results, on dry leaves of Dipterocarpaceae considered problematic. This research aims to find a suitable method for extracting from preserved leaves. Preserved silica gel will up, making them tough destro...

Plasmid DNA chromatography is a powerful field in constant development and evolution. The use of this technique considered mandatory the production an efficient safe formulation to be applied for plasmid-mediated gene therapy. Concerning this, search ideal chromatographic support/ligand combination motivated scientist pursue continuous improvement on plasmid performance, looking progression lig...

DNA vectors for human gene therapy have to meet the efficacy and safety requirements. Minicircles (MCs), a class of optimized DNA vectors free of plasmid backbone (PB) DNAs, have emerged as promising candidates because of their superior transgene expression profiles. However, the existence of impure DNAs, including the unrecombined MC producing plasmid (PP) and PB circle, in the MC products mad...

The ability to amplify genomic DNA in a polymerase chain reaction (PCR) is dependent upon the purity of the DNA template. Environmental genomic DNA often contains contaminants (e.g., polyphenols, humic acids, polysaccharides) that reduce template purity and can be difficult to remove, thereby inhibiting PCR amplification. There is thus a need for a method to purify extracted genomic DNA without...