نتایج جستجو برای: dna dna hybridization

تعداد نتایج: 543321  

Journal: :Chemosensors 2023

Tumor cell-derived exosomes are considered a potential source of cancer biomarkers. Here, we developed an electrochemical sensing platform for the rapid and simple detection exosomes, using CCRF-CEM exosome as model. The utilizes cyclic nicking enzyme cleavage hybridization chain reaction (HCR) dual-signal amplification. A hairpin aptamer probe (HAP) containing was designed assay. specific bind...

ژورنال: علوم آب و خاک 2009
باقی زاده, امین, خنامانی فلاحتی پور, سوده, شاهسوند حسنی , حسین, کریم زاده, قاسم,

The genomic in situ hybridization (GISH) has been used to identify euploidy and aneuploidy in segregation generations of various plants. In this study, the GISH with minor modifications including, slide preparation of putative secondary Tritipyrum (F2) root meristemic cells, labeled genomic DNA of Thinopyrum bessarabicum by fluorescein 12-dUTP nucleotide as probe, genomic DNA of Thinopyrum bess...

باقی زاده, امین, خنامانی فلاحتی پور, سوده, شاهسوند حسنی , حسین, کریم زاده, قاسم,

The genomic in situ hybridization (GISH) has been used to identify euploidy and aneuploidy in segregation generations of various plants. In this study, the GISH with minor modifications including, slide preparation of putative secondary Tritipyrum (F2) root meristemic cells, labeled genomic DNA of Thinopyrum bessarabicum by fluorescein 12-dUTP nucleotide as probe, genomic DNA of Thinopyrum bess...

استوری, کریستوفر, حاجیا, مسعود,

Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this...

Journal: :hepatitis monthly 0
yongcan guo the key laboratory of laboratory medical diagnostics, ministry of education, chongqing medical university, chongqing, china shangchun sheng the key laboratory of laboratory medical diagnostics, ministry of education, chongqing medical university, chongqing, china bin nie the key laboratory of laboratory medical diagnostics, ministry of education, chongqing medical university, chongqing, china zhiguang tu the key laboratory of laboratory medical diagnostics, ministry of education, chongqing medical university, chongqing, china; the key laboratory of laboratory medical diagnostics, ministry of education, chongqing medical university, chongqing, china. tel: +86-2368485759, fax: +86-2368485239

background hepatitis b virus (hbv) covalently closed circular dna (cccdna) served as a vital role in the life cycle of the virus and persistent infection. however, specific and quantitative methods for cccdna detection have not been available. objectives our aim was to develop and primarily evaluate a quantitative method for hbv cccdna based on magnetic capture hybridization and quantitative pc...

Journal: :cell journal 0

objective: electroporation is the most common method used for the transfection of dna. although this method has been attributed for various cell using different buffer compositions, the effects of dna concentration on the transfection efficiency has not yet been studied. here the correlation between the efficiency of electroporation reaction and increments of dna concentration has been investig...

اجلی, محسن, بدلی, حمید, حمزه‌یی, حسین, رهنما, مهدی,

Background and Objective: Epidemiological studies indicate that not only the incidence of fungal infections is dramatically on the rise, especially in the immunocompromised hosts, but also the sensitivity of etiological agents to antifungal drugs shows a remarkable reduction. Therefore, early detection at the species level is critically important for proper clinical management. Because standard...

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