In the cell cycle, the disruption of a checkpoint control mechanism for G2/M phase which monitors DNA damages is one of the triggers for oncogenic transformation. The major event of this mechanism is the p53/Mdm2 signaling pathway-mediated repression of M-phase Promoting Factor (MPF) activation. With the occurring some DNA damages, the protein levels of the p53/Mdm2 shows the oscillation, and t...

Background and Aims: Nickel oxide nanoparticles (NiO-NPs) are one of the most used nanoparticles, especially as photosensitizers. Although some studies evaluate their toxicity in liver, information about at cellular molecular levels is still controversial. In present study, it was aimed to investigate vitro toxic potentials NiO-NPs (average size 15.0 nm) liver (HepG2) cell line. Methods: were ...

The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of al...

diseases. These include cancer, Parkinson’s disease, Alzheimer’s disease, atherosclerosis, heart failure, myocardial infarction, Schizophrenia, bipolar disorder, fragile X syndrome, sickle cell disease and chronic fatigue syndrome. Oxidative stress is likely to be involved in agerelated development of cancer. The reactive species produced in oxidative stress can cause direct damage to the DNA a...

Ionizing radiation induces bistranded clustered damages--two or more abasic sites, oxidized bases and strand breaks on opposite DNA strands within a few helical turns. Since clusters are refractory to repair and are potential sources of double-strand breaks (DSBs), they are potentially lethal and mutagenic. Although induction of single-strand breaks (SSBs) and isolated lesions has been studied ...

Introduction: Radiotherapy is still one of the main options for cancer treatment but it is in association with damage to normal cells as well as the tumor cells. To reduce the injury in normal cells we have evaluated the effect of 528 hertz sound after X irradiation in peripheral blood lymphocytes. Materials and Methods: in this study, peripheral blood was o...

Introduction: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high dose rate brachytherapy (BR).   Materials and Methods: DU-145 cells were cultured as spheroids ...

Clustered damages are formed in DNA by ionising radiation and radiomimetic anticancer agents and are thought to be biologically severe. 7,8-dihydro-8-oxoguanine (8-oxoG), a major DNA damage resulting from oxidative attack, is highly mutagenic leading to a high level of G.C-->T.A transversions if not previously excised by OGG1 DNA glycosylase/AP lyase proteins in eukaryotes. However, 8-oxoG with...