Nuclear DNA content variation over orders of magnitude across species has been attributed to 'junk' repetitive DNA with limited adaptive significance. By contrast, our previous work on Silene latifolia showed that DNA content is negatively correlated with flower size, a character of clear adaptive relevance. The present paper explores this relationship in a broader phylogenetic context to inves...

BACKGROUND AND AIMS Gossypium is an economically important, globally distributed taxon comprising more than 50 species. DNA content estimates from about half of the species indicate over a 3-fold variation exists. However, the nine DNA content estimates for G. hirsutum reveal over a 2-fold difference for this species alone. Recent reports have shown that several plant compounds can bias DNA con...

The number of pore complexes per nucleus was determined for a wide variety of cultured cells selected for their variable DNA content over a range of 1-5,6000. The pore number was compared to DNA content, nuclear surface area, and nuclear volume. Values for pore frequency (pores/square micrometer) were relatively constant in the species studied. When the pore to DNA ratio was plotted against the...

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. W...

in this study, freshwater fish cyprinus carpio was exposed to sub lethal concentration of synthetic pyrethroids insecticide cypermethrin to evaluate the impact on nucleic acids in different organs. fishes were exposed to sub lethal concentration (1/10th of lc50 value) of cypermethrin for three different durations, 7, 14 and 21 days. in seven day exposed fishes, dna and rna contents were not alt...

• A DNA molecule consists of two strands, commonly referred to as top and bottom; positive and negative; or normal and reverse. The contents of one strand form a reverse complement of the other strand. The ”complement” part: A(denine) is always paired with Thymine, while Cytosine is always paired with Guanine. The ”reverse” part: the strand are read in opposite directions: the 5’ end of one str...