background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...

Geminivirus replication initiator protein (Rep) is a multifunctional viral required for replication. During the process of replication, Rep acts as site- and strand-specific endonuclease, ligase, ATPase, helicase. B' motif β-hairpin loop geminivirus are conserved important Rep-mediated helicase activity To dissect roles various amino acid residues in its unwinding DNA, we investigated eight nea...

Background: Coumarins comprise a large family of heterocyclic compounds with benzo-a-pyrone moiety. Objectives: This study aimed to analyze the binding affinity 3-(1H-tetrazol-5-yl) coumarin bovine serum albumin (BSA) and calf thymus DNA (Ct-DNA) using fluorescence spectroscopy. The quenching was recognized during interaction between BSA, followed by static mechanism. Methods: hydrogen bonds, h...

Abstract Abnormally formed FUS/EWS/TAF15 (FET) fusion oncoproteins are essential oncogenic drivers in many human cancers. Interestingly, at the molecular level, they also form biomolecular condensates specific loci. However, how these lead to gene transcription and features encoded DNA element regulate condensate formation remain unclear. Here, we develop an vitro single-molecule assay visualiz...

Cell to communication is the most important process in all living organisms. This network essential for growth, differentiation, development and metabolic functions. As plant cell has a rigid wall so there are some special ways using through structure called Plasmodesmata. The messages from DNA of dividing cells pass signals or other RNA molecules. But molecules cannot independently cells, prot...

Significance In transcription factors’ search for target genes, one-dimensional diffusion of the protein along DNA is essential. Experimentally, it remains challenging to resolve individual diffusional steps on DNA. Here, we report mainly all-atom equilibrium simulations a WRKY domain in association with and We demonstrate complete stepping cycle one base pair within microseconds, stochastic mo...