Supplemental Experimental Procedures Cell culture. Rat bone marrow cells (BMC) were cultured in αDMEM culture medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum. Rat primary ear fibroblasts (PEF) were cultured in DMEM culture medium (Invitrogen) supplemented with 10% fetal bovine serum. Rat iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) in K...

Activated macrophage phenotypes were influenced by the culture medium; a murine macrophage-like cell line, J774.1/JA-4, expresses different activated-macrophage phenotypes induced by lipopolysaccharide (LPS) and/or interferon-γ (IFN-γ) when the cells are incubated in either Ham’s F-12 medium (F-12) or Dulbecco’s modified Eagle medium (DMEM). Among these phenotypes, NO production and iNOS expres...

BACKGROUND Despite the regenerative potential of the peripheral nervous system, severe nerve lesions lead to loss of target-organ innervation, making complete functional recovery a challenge. Few studies have given attention to combining different approaches in order to accelerate the regenerative process. OBJECTIVE Test the effectiveness of combining Schwann-cells transplantation into a biod...

BACKGROUND Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC) are human intestinal pathogens responsible for diarrhea in both developing and industrialized countries. In research laboratories, EPEC and EHEC are defined on the basis of their pathogenic features; nevertheless, their identification in routine laboratories is expensive and laborious. Therefore, the aim of the prese...

The object of this study was to identify the possible effects of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocyte complexes (COC’s) matured and fertilized in vitro in two serum free media Dulbecco’s modified Eagles medium (DMEM) and Ham’s F-10. Oocytes were matured in vitro for 24 h either...

Background: Gingival tissue and periodontal ligament act as sources of mesenchymal stem cells (MSCs) that play a vital role in regeneration, but they both have limitations for cell availability. MSCs cultivated expanded various media formulations could be used basis the development therapy protocols. Purpose: This study aimed to determine optimum culture formulation cultivation expansion human ...

Cell Cultures Derivatives of the NCCIT teratocarcinoma cell line were cultured in Dulbecco's modified eagle medium (DMEM) (Lonza BioWhittaker, Basel, Switzerland), containing 2 mM glutamine and 4.5 g/L glucose. Media was supplemented with 10% fetal bovine serum (FBS) (Gibco-Invitrogen Corporation, Carlsbad, CA, USA). Cell cultures were maintained at 37 ̊C with 5% CO2 in a humidified atmosphere. ...

Glioblastoma stem cells are able to reform original glioblastoma and express the neural stem cell marker CD133 and Nestin. They can self-renew and proliferate in tumor sphere medium containing EGF, bFGF and LIF that is known to be permissive for stem cell proliferation. In this study, we found that neurosphere-like colonies appeared after the human primary glioblastoma cells had been switched i...

It was previously shown that serum low density lipoprotein (LDL) binds to type I collagen gels and that the binding is increased after modification by cultured endothelial cells. It is now demonstrated that when LDL is incubated with cells cultured in Dulbecco's modified minimal essential medium (DMEM), the subsequent binding of LDL to collagen is considerably less than after incubation with en...