In the kidney, L-ornithine is reabsorbed along the proximal convoluted tubule (PCT), transported by basolateral carriers, and produced by arginase II (AII). Here, the renal metabolic fate of L-ornithine was analyzed in male and female rats. Kidneys and renal zones were dissected and used for Western blot analysis, immunofluorescence, and electron microscopic studies. Ornithine aminotransferase ...

We reviewed the literature on ornithine supplementation and related topics. Nutritionists and physicians have reported that ornithine supplementation is useful. Paediatricians and biochemists have reported that ornithine is supplemented for NH3 detoxification in the hyperornithinaemia–hyperammonaemia–homocitrullinuria (HHH) syndrome. In contrast, ophthalmic researchers have reported retinotoxic...

AIM to determine the effect of L-ornithine-L-aspartate (LOLA) together with the nutritional improvement and branched chain amino acids (BCAAs) substitution, on encephalopathy in liver cirrhosis with malnutrition. METHODS liver cirrhosis patients visited Cipto mangunkusumo Hospital in June-October 2009 were evaluated by critical flicker frequency (CFF) test. Encephalopathy is defined when CFF ...

In cultured mammary explants from both virgin and midpregnant mice which contained a low and a high initial level of putrescine, respectively, addition of an ornithine decarboxylase inhibitor, DL-a-hydrazino-Saminovaleric acid at the onset of culture prevented an early, “osmolarity-sensitive” rise in the putrescine level that occurred prior to induction of DNA synthesis by insulin. In the pres...

Effects of L-ornithine (150 mg/l) on the germination, seedling growth, mitotic index, chromosome aberrations and micronucleus frequency Allium cepa L. bulbs germinated at 0.125 M salinity were studied. The radicle number group III in medium with ornithine alone as compared to ones I (control) which distilled water medium. But, their germination percentage, length fresh weight statistically same...

I.c.v. injection of L-ornithine has been shown to have sedative and hypnotic effects on neonatal chicks exposed to acute stressful conditions. To clarify the mechanism, we conducted three experiments under strengthened stressful conditions with corticotropin-releasing factor (CRF). In Experiment 1, the effect of i.c.v. injection of CRF, L-ornithine (0.5 μmol) or CRF with L-ornithine on the stre...

Phaseolotoxin, a tripeptide inhibitor of ornithine transcarbamoylase, is a phytotoxin produced by Pseudomonas syringae pv. phaseolicola, the causal agent of halo-blight in beans. In vivo the toxin is cleaved to release N6-(N'-sulpho-diaminophosphinyl)-Lornithine, the major toxic chemical species present in diseased leaf tissue. This paper reports on the interaction between N8-(N'-sulpho-diamino...

In primary cultures of adult rat hepatocytes, the relative rate of ornithine aminotransferase synthesis is increased approximately 7- to 8-fold in response to dibutyryl cyclic AMP. The corresponding increase in ornithine aminotransferase messenger RNA under these conditions was observed to be only 2- to 3-fold. The levels of ornithine aminotransferase mRNA were measured by hybridization of cell...

Pseudomonas putida P2 (ATCC 25571) grown on r,-lysine or DL-pipecolic acid is induced for a high velocity lysine transport system. Double reciprocal plots of L-lysine transport in induced cells are linear. K, and V,,, values are 7.3 X 10V6 M and 130 nmoles per mm per mg for lysinegrown cells and 9.3 X 10e6 M and 42 mnoles per min per mg for cells grown on pipecolate. Lysine transport is optimal...

We isolated a novel gram-positive bacterium, Brevibacillus texasporus, that produces an antibiotic, BT. BT is a group of related peptides that are produced by B. texasporus cells in response to nutrient limitation. We report here purification and determination of the structure of the most abundant BT isomer, BT1583. Amino acid composition and tandem mass spectrometry experiments yielded a parti...