A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld X Mus spretus)F1 X C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP charac...

Using granzyme B-deficient mice obtained by gene targeting, we previously demonstrated that granzyme B is required for the rapid induction of apoptotic target cell death by cytotoxic T lymphocytes (CTLs); however, CTLs are also equipped with additional effector mechanisms. In the present study, we examined the mechanisms responsible for granzyme B-independent cytotoxicity using in vitro lytic a...

The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathway is set in the female mode (terminal fem/fog genes inactive), gld-1 (null) g...

Germline stem cell differentiation in Caenorhabditis elegans is controlled by glp-1 Notch signaling. Cell fate regulator GLD-1 is sufficient to induce meiotic entry and expressed at a high level during meiotic prophase, inhibiting mitotic gene activity. glp-1 signaling and other regulators control GLD-1 levels post-transcriptionally (low in stem cells, high in meiotic prophase), but many aspect...

This paper deals with generalized low-density (GLD) lattices which have been recently shown to be an excellent family of lattices for communication over the Gaussian channel. Under iterative decoding, numerical results for GLD lattices show an error rate per lattice coordinate extremely close to Poltyrev theoretical limit of an infinite constellation. These results are found for GLD ensembles w...

Under normality and homoscedasticity assumptions, Linear Discriminant Analysis (LDA) is known to be optimal in terms of minimising the Bayes error for binary classification. In the heteroscedastic case, LDA is not guaranteed to minimise this error. Assuming heteroscedasticity, we derive a linear classifier, the Gaussian Linear Discriminant (GLD), that directly minimises the Bayes error for bina...

Current works on process-oriented tolerancing for multi-station manufacturing processes (MMPs) have been mainly focused on allocating fixture tolerances to ensure part quality specifications at a minimum manufacturing cost. Some works have also included fixture maintenance policies into the tolerance allocation problem since they are related to both manufacturing cost and final part quality. Ho...

Tolerancing decisions can profoundly impact the quality and cost of electro-mechanical assemblies. Existing approaches to tolerance analysis and synthesis in design entail detailed knowledge of geometry of the assemblies and are mostly applicable during advanced stages of design, leading to a less than optimal design process. During the design process of assemblies, both the assembly structure ...

The Gld gene of Drosophila melanogaster is transiently expressed during every stage of development. The temporal pattern of Gld expression is highly correlated with that of ecdysteroids. Exogeneous treatment of third instar larvae with 20-hydroxyecdysone induces the accumulation of Gld mRNA in the hypoderm and anterior spiracular gland cells. During metamorphosis Gld is expressed in a variety o...

Maintenance of the stem cell population in the C. elegans germline requires GLP-1/Notch signaling. We show that this signaling inhibits the accumulation of the RNA binding protein GLD-1. In a genetic screen to identify other genes involved in regulating GLD-1 activity, we identified mutations in the nos-3 gene, the protein product of which is similar to the Drosophila translational regulator Na...