In recent years, substantial advances have been made in the development of artificial metalloenzymes. These hybrid bioinorganic catalysts seen their selectivity improved through techniques including site-directed and site-saturation mutagenesis directed evolution. Their scope has expanded by introduction various abiological metal catalysts, new anchoring strategies designs to enhance reusabilit...

site-directed mutagenesis (sdm) as a powerful technique was used to change two important and conserved amino acids in 5-enolpyruvylshikimate 3- phosphate synthase (epsps) gene of e. coli. the mutations changed glycine 96 to alanine and alanine 183 to threonine. these two amino acids are very important for intraction of the wide spectrum herbicide, glyphosate, to epsp synthase enzymes. by design...

Experimental results are presented suggesting that 23S rRNA is directly involved in the peptide bond formation usually performed on the ribosome. Although several reports have indicated that the eubacterial peptidyltransferase reaction does not necessarily require all the ribosomal proteins, the reconstitution of peptidyltransferase activity by a naked 23S rRNA without the help of any of the ri...

With the development of recombinant DNA technology and the many analytic techniques based on restriction endonucleases, it has become relatively routine to isolate a segment of genomic DNA carrying a gene of interest, to define the limits of the gene within this DNA segment, and then to determine the gene's complete nucleotide sequence. In this way, a number of structural questions can be direc...

Fig 4 is incorrect. Fig 4 incorrectly appears a duplicate of Fig 5. The authors have provided a corrected version of Fig 4 here. Fig 4. Structures of site-directed amino acid residues with other vicinal residues by H-bond. Copyright: © 2015 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distrib...