We automated the Jendrassik-Grof method for measurement of bilirubin with the Greiner Selective Analyzer GSA II D. Comparison with the manual Jendrassik-Grof method showed a highly significant correlation coefficient of 0.994 for total and 0.980 for "direct" bilirubin measurement. The automated method showed good precision and was linear to 667 mu mol/L, the highest concentration tested. Additi...

In eight unique challenges mailed by The College of American Pathologists Comprehensive Chemistry Survey to participating laboratories within 3 years, results for direct-reacting bilirubin (DBIL) were highly variable among the 12 largest peer groups, and most of the results differed greatly from the values obtained by a referred method. Peer-group mean values for total bilirubin (TBIL) were in ...

background: hyperbilirubinemia is considered one of the most prevalent problems in newborns. phototherapy, exchange transfusion, and herbal medicine are common therapeutic approaches for preventing any neurologic damage in infants with neonatal jaundice. however, herbal medicine is less commonly used. aim: this study aimed to investigate the effect of bilineaster drop on neonatal hyperbilirubin...

BACKGROUND A reliable indicator of cholestasis is the presence of abnormal concentrations of bilirubin mono- and diglucuronide [conjugated bilirubin (CB)] in blood. A routine assay of CB is available only to those who possess a certain type of clinical analyzer. We describe a two-point manual method for CB that could be adapted as a rate assay to automated clinical analyzers. METHODS The meas...

  Background: Delay in diagnosis and treatment of perforated appendicitis may cause life-threatening complications. The aim of this study was to determine and compare pre-operative total and direct bilirubin levels in cases of simple and perforated acute appendicitis in order to improve the clinical decision making.   Methods: This prospective observational study included eighty patients who ...

background: routine repeat testing of critical laboratory values is very common these days to increase their accuracy and to avoid reporting false or infeasible results. we figure that repeat testing of critical laboratory values has any benefits or not.   methods : we examined 2233 repeated critical laboratory values in 13 different hematology and chemistry tests including: hemoglobin, white b...

Background and Purpose: Previous studies have suggested that bilirubin is a potential contributor to cerebral vasospasm. The purpose of this investigation was to determine whether bilirubin accrues in subarachnoid clot, whether its vasoconstrictive effect could involve a direct action on arterial smooth muscle cells, and, if so, whether bilirubin affects their Ca2+ uptake. Methods: Subarachnoid...

BACKGROUND AND PURPOSE Previous studies have suggested that bilirubin is a potential contributor to cerebral vasospasm. The purpose of this investigation was to determine whether bilirubin accrues in subarachnoid clot, whether its vasoconstrictive effect could involve a direct action on arterial smooth muscle cells, and, if so, whether bilirubin affects their Ca2+ uptake. METHODS Subarachnoid...

BACKGROUND The association between serum bilirubin levels and incident chronic kidney disease (CKD) in the general population is unknown. We aimed to examine the association between serum bilirubin concentration (total, direct, and indirect) and the risk of incident CKD. METHODS AND FINDINGS Longitudinal cohort study of 12,823 Korean male workers 30 to 59 years old without CKD or proteinuria ...

An artificial liver support system for plasma exchange and plasma perfusion through BR-601 resin using a membrane separator was applied to 5 patients with postoperative liver failure. Percent absorption of total and direct bilirubin, and of bile acids were 77.1 +/- 6.4, 78.4 +/- 6.1, and 93.4 +/- 3.6%, respectively, when 250 ml of plasma was treated. Percent reductions in total and direct bilir...