The phenomenological model of Khossravi and Connors (1992) has been adopted to calculate the electrophoretic mobility of drugs at different concentrations of solvents in a binary mixture. The accuracy and predictability of the model have been evaluated employing 14 experimental data sets by using average percentage mean deviation (APMD). The obtained APMD for correlative and predictive studies ...

We write the Euler characteristic χ(G) of a four dimensional finite simple geometric graph G = (V,E) in terms of the Euler characteristic χ(G(ω)) of two-dimensional geometric subgraphs G(ω). The Euler curvature K(x) of a four dimensional graph satisfying the Gauss-Bonnet relation ∑ x∈V K(x) = χ(G) can so be rewritten as an average 1 − E[K(x, f)]/2 over a collection two dimensional “sectional gr...

The mitochondrial polypeptides from maize lines susceptible and resistant to Helminthosporium maydis were studied by single- and two-dimensional gel electrophoresis. Approximately 120 polypeptides were detected by two-dimensional gel electrophoresis. However, it was not possible to detect qualitative differences between the mitochondrial polypeptides of the two inbreds. These observations are d...

Ouchterlony immunodiffusion of sonicated Vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. Antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. Tandem two-dimensional immunoelectrophoretic analyses of V. vulnificus antigens from various strains ...

Consistent differences in expression of specific proteins were observed between numerous cancer cell clones with high and low potential for spontaneous metastasis. Seventeen clones from two unrelated murine fibrosarcomas were examined concomitantly for spontaneous formation of lung metastases and for occurrence of individual polypeptide differences by high resolution two-dimensional gel electro...

We have used two-dimensional gel electrophoresis to study the structural transition to the triplex H form of sequences 5'-AAGGGAGAAXGGGGTATAGGGGYAAGAGGGAA-3' where X and Y are any DNA bases. The transition was observed at acid pH under superhelical stress. For X = Y = A or X = Y = G the sequences corresponded to homopurine-homopyrimidine mirror repeats (H-palindrome) which are known to adopt th...

Quantitative immunoelectrophoresis allows a polyvalent approach to immuno-chemical problems, i.e., identification, quantitation, and thereby standardization of antigens and antibodies in complex mixtures, for example as in crude extracts of microorganisms and their corresponding antisera. This approach is a short cut to conventional standardization of single purified substances, since the polyv...

Five arginine-utilizing, nonglycolytic Mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. The survey included: Mycoplasma hominis, Mycoplasma arthritidis, Mycoplasma gateae, Mycoplasma gallinarum, and three strains of Mycoplasma arginini. Although the M. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which...

We compared male-reproductive-tract polypeptides of Drosophila melanogaster and D. simulans by using two-dimensional gel electrophoresis. Approximately 64% of male-reproductive-tract polypeptides were identical between two randomly chosen isofemale lines from these two species, compared with 83% identity for third-instar imaginal wing-disc polypeptides. Qualitatively similar differences were fo...

Two Azotobacter vinelandii strains capable of growing on N2(Nif+) were isolated from two different mutant strains that lacked dinitrogenase activity (Nif-). Extracts of N2-grown cells of the two Nif+ strains lacked significant amounts of the "conventional" dinitrogenase protein subunits, as determined by two-dimensional gel electrophoresis. Instead, the extracts contained at least four new prot...