BACKGROUND Glioblastoma Multiforme (GBM) or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. Several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications. Proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. METHODS In this study protei...

ethanol known as ethyl alcohol is being widely used around the world. many serious diseases are related with its consumption.  alcohol posses many divers effects on human body including risk of cirrhosis and/or hepatocellular carcinoma. therefore, analysis of this component is prominent. fibroblast cells were cultured in various dosages of ethanol. the effective dosage was then investigated by ...

Aim Detection of protein expression changes in human cystic echinococcosis sera by 2D gel electrophoresis. Background Diagnosis and successful treatment of cystic echinococcosis (CE) is a major challenge, up to now. Identification of related expressed proteins using proteomics tools and bioinformatics analysis of patients' sera have not been investigated, so far. Methods Sera from eight con...

BACKGROUND Although highly protective, cardiac surgery using cardioplegia and cardiopulmonary bypass (CP/CPB) subjects myocardium to hypothermic reversible ischemic injury that can impair cardiac function which results in a greatly enhanced risk of mortality. Acute changes in myocardial contractile activity are likely regulated via protein modifications. We performed the following study to dete...

background hepatocellular carcinoma (hcc), a major fatal cancer worldwide, is induced by different etiological factors in the liver. objectives to gain insight into serum protein profiling of hcc with different etiologies. patients and methods we subjected the sera of hbv-hcc, hcv-hcc, non-b non-c-hcc patients, and healthy volunteers to two-dimensional gel electrophoresis (2-de) and liquid chro...

BACKGROUND Proteomic analysis is emerging as a highly useful tool in food research, including studies of food allergies. Two-dimensional gel electrophoresis involving isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis is the most effective method of separating hundreds or even thousands of proteins. In this study, albumin and globulin tractions of pea seeds cv. R...

saprophitic pseudomonas species are root-colonizing bacteria that can improve plant health. efficient exploitation of these bacteria in agriculture requires knowledge of traits that enhance ecological performance in the rhizosphere. some pseudomonas fluorescens strains present biocontrol properties, protecting the roots of some plant species against plant pathogens. these bacteria induce system...

To investigate the proteomic background of the carcinogenesis and progression of pancreatic cancer, the protein expression profiles of nine well-characterized pancreatic adenocarcinoma cell lines, whose metastatic potential was previously examined in a mouse xenograft model, and two immortalized pancreatic duct cell lines were examined. Two-dimensional difference gel electrophoresis (2D-DIGE) i...

Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks, artifacts and noise. Watershed transform is one of the common methods for image segmentation. Nevertheless, in 2DGE image segmentation, the no...