Fructose-1,6-bisphosphate aldolase plays an effective role in glucose metabolism and gluconeogenic pathway, and reversibly catalyzes the split of fructose 1,6-bisphosphate into the triose phosphates D-glyceraldehyde phosphate and dihydroxyacetone phosphate. Aldolase has 160 kDa molecular weight and three tissue specific isozymes. Gestational diabetes mellitus is defined as glucose intolerance t...

The phenacylimidazolium compound LY 177507 was shown by Harris et al. (Harris, R. A., Yamanuchi, K., Roach, P. J., Yen, T. T., Dominiani, S . J., and Stephens, T. w. (1989) J. Biol. Chem. 264,14674-14680) to stimulate glycogen synthesis greatly in isolated rat hepatocytes. We extended studies with this compound, designated proglycosyn (Yamaguchi, K., Stephens, T. W., Chikadar, K., Depaoli-Roach...

Rat liver microsomes were used as an enzyme source to study dietary-induced changes in the rate of dihydroxyacetone phosphate esterification. Rats were fed (1) 75% glucose or fructose diets for various time intervals, or (2) fed a fractose diet for six days and then a chow diet. Both the glucose and fructose diets produced a 2--3-fold increase in total and neutral glycerolipid formation from di...

The glycerol portion of lipids may be derived biosynthetically by reduction of dihydroxyacetone phosphate to glycerolphosphate, and then be acylated with fatty acids or, alternatively, dihydroxyacetone phosphate may first be acylated and then reduced to 1-acyl sn glcerol-3-phosphate. Since the former pathway utilizes NADH for reduction of the C-2 carbonyl, while the latter requires NADPH, we we...

The enzyme NADPH :acyl or NADPH:alkyl dihydroxyacetone phosphate oxidoreductase has been purified from Ehrlich ascites cell microsomes and guinea pig liver mitochondria. The membrane bound enzyme was extracted by the treatment of the particles with a sodium deoxycholate-KClNADPH solution and purified by chromatography on either a Sephadex G-ZOO or a Sepharose 4B column. The enzyme from ascites ...

Microsomal enzymes from Ehrlich ascites tumors synthesized 0-alkyl glyceryl ethers from hexadecanol and dihydroxyacetone phosphate in the presence of ATP, CoA, and Mg++. Glyceryl ether synthesis occurred via the formation of 0-alkyldihydroxyacetone phosphate and O-alkyldihydroxyacetone. Although NADPH inhibited the initial reaction of dihydroxyacetone phosphate with fatty alcohol, it was requir...