Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of larger 1D gel-derived gel bands with liquid handling work stations is less we...

a method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. the homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. a sample then received a new way of pre-treatment, called microwave digestion, before uv-vis spec...

This paper builds a quantitative thermodynamic model for the microbial hydrolysis process (MHP, which uses Caldicellulosiruptor bescii at 75°C pre-digestion) producing biogas from 5-10% aqueous suspension of dairy manure (naturally buffered near pH 7.8 by ammonium bicarbonate) anaerobic digestion with mix acetoclastic and syntrophic methanogenesis. Standard Gibbs energy changes were calculated ...

Background & Aims of the Study: Sarcocystiosis is a zoonosis appeared in domestic animals caused by various species of Sarcocystis. This protozoan disease has worldwide distribution among human and many species of animals. Humans acquire infection by eating of raw and under cooked beef, pork or mincemeat containing schizonts of Sarcocystis hominis and S. suihominis. The aim of present study is ...

background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...