The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of the conformational transition of metmyoglobin-heparin complex equilibrated at ...

Thermosensitive nanoparticles were prepared via the self-assembly of two different poly(ε-caprolactone)-based block copolymers of poly(N-isopropylacrylamide)-b-poly(ε-caprolactone) (PNPCL) and poly(ethylene glycol)-b-poly(ε-caprolactone) (PEGCL). The self-aggregation and thermosensitive behaviors of the mixed nanoparticles were investigated using 1H-NMR, turbidimetry, differential scanning micr...

A review of the relevant literature on 1-alkyl-1-methylpyrrolidinium-based ionic liquids has been presented. The phase diagrams for the binary systems of {1-ethyl-1-methylpyrrolidinium trifluoromethanesulfonate (triflate) [EMPYR][CF(3)SO(3)] + water, or + 1-butanol} and for the binary systems of {1-propyl-1-methylpyrrolidinium trifluoromethanesulfonate (triflate) [PMPYR][CF(3)SO(3)] + water, or...

The adsorption of CO on polycrystalline TiO2 was investigated by static adsorption microcalorimetry. The initial differential heat of adsorption (qdiff,0) of CO on polycrystalline titania is 40 kJ/mol, and the standard adsorption entropy (Deltas0) is -104 J mol(-1) K(-1). These results are consistent with those derived from temperature-programmed desorption and FTIR results in the literature. T...

In the reaction of 4,4'-methylenebis(phenyl isocyanate), polycaprolactone diol, and N-methyldiethanolamine, they were synthesized aqueous dispersions of polyurethane cationomers, from which films were prepared after adding 0-2 wt% graphene. In order to obtain nanocomposites, graphene was previously noncovalent functionalized in tetrahydrofurane in the field of ultrasound. The chemical structure...

Differential scanning microcalorimetry (DSC) and UV–VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat’ after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum ...

As detected by adiabatic differential scanning microcalorimetry, [2-(alkyloxy)-phenyl]-2-(1-piperidinyl)ethyl esters of carbamic acid (CnPPEECA, n is the number of carbon atoms in the alkyloxy substituent) with local anesthetic and antiarrhythmic activities interact with 1,2-dipalmitoyl-sn-glycero-3-[phosphorac-(1-glycerol)] model membranes (DPPG). CnPPEECAs form solid-like solutions with DPPG ...

Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followed by different...

The enzyme 1-aminocyclopropane-1-carboxylate deaminase (ACCD) converts ACC, the precursor of the plant hormone ethylene, to alpha-ketobutyrate and ammonium. This enzyme has been identified in soil bacteria and has been proposed to play a key role in microbe-plant association. A soluble recombinant ACCD from Pseudomonas putida UW4 of molecular weight 41 kDa has been cloned, expressed, and purifi...