The proliferation and increased use of genetically manipulated rodents are taxing laboratory animal facilities and diagnostic laboratories. Although serological testing remains the standard for monitoring immunologically intact animals, molecular diagnostics are playing increasing roles in detecting infections in animals that have compromised immune systems. The importance of these assays is pa...

The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant protei...

A mandatory objective of any control programme aiming at eradicating BVD from an infected bovine population is the removal of animals persistently infected (PI) with BVDV. In practice this will require a selection of reliable diagnostic assays. Approaches on how to identify PI animals can be indirect, by first identifying herds with a high probability of containing PI animals through diagnostic...

background: helicobacter pylori (hp) is a gram negative, spiral shaped bacterium which colonizes the gastric mucosa and induces gastroduodenal complications varying from mild gastritis with no clinical complications to peptic ulcer diseases and even gastric malignancies. the rate of hp infection is 30-50% in developed countries whereas it has been rated up to 80% of the adult population in deve...

OBJECTIVE Accurate laboratory testing is a critical component of dengue surveillance and control. The objective of this programme was to assess dengue diagnostic proficiency among national-level public health laboratories in the World Health Organization (WHO) Western Pacific Region. METHODS Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a pro...

Complications in managing the dengue virus infections include the lack of rapid diagnostic procedures and at the same time the symptoms of dengue infection are often confused with those of other diseases. Two commercial rapid serological diagnostic kit methods (Dengue Day 1 test, J Mitra and Co. Pvt. Ltd., New Delhi, ImmunoComb II Dengue IgM/IgGBispot kit (Orgenics Pvt. Ltd., Israel)were evalua...

BACKGROUND A novel porcine deltacoronavirus (PDCoV), also known as porcine coronavirus HKU15, was reported in China in 2012 and identified in the U.S. in early 2014. Since then, PDCoV has been identified in a number of U.S. states and linked with clinical disease including acute diarrhea and vomiting in the absence of other identifiable pathogens. Since PDCoV was just recently linked with clini...

In this issue of Clinical and Vaccine Immunology, Siev and colleagues present an evaluation of antibody responses to four immunodominant proteins of Mycobacterium tuberculosis in patients with HIV-associated pulmonary tuberculosis (TB) in South Africa (M. Siev, D. Wilson, S. Kainth, V. O. Kasprowicz, C. M. Feintuch, E. Jenny-Avital, and J. J. Achkar, 21:791-798, 2014, doi:http://dx.doi.org/10.1...